Haloperidol attenuates β-amyloid-induced calcium imbalance in human fibroblasts

A. Palotás, B. Penke, M. Palotás, A. Sz Kenderessy, L. Kemény, E. Kis, G. Vincze, Z. Janka, J. Kálmán

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Antipsychotics are widely used in the treatment of behavioral and psychological symptoms of dementia. A low frequency of Alzheimer's disease in patients with schizophrenia is reported, and it has been proposed that antipsychotic medications, such as haloperidol, may be responsible. Disruption of intracellular calcium levels is considered to play a key role in β-amyloidinduced neurotoxicity in Alzheimer's disease. Haloperidol has also been reported to interact with calcium homeostasis through dopamine-2 and sigma-1 receptors, and other, yet unknown mechanisms. Objective: Therefore, we investigated whether differences in the basal intracellular free calcium levels of cultured cutaneous fibroblasts - cells that do not express dopamine-2 and sigma-1 receptors - derived from sporadic Alzheimer patients and from age-matched control individuals after haloperidol treatment might be present. Methods: Intracellular calcium level was measured in Fura-2AMloaded human fibroblasts by dual wavelength spectrofluorimetry. Results: Alzheimer cells exhibited significantly lower calcium level as compared to the control cultures. Exposure of fibroblasts to β-amyloid peptide resulted in increased calcium concentration of the control cells, but not of Alzheimer fibroblasts. Co-incubation of cultures with a therapeutic dose of haloperidol blocked the β-amyloid-induced elevation of calcium. Conclusion: This finding indicates that haloperidol efficiently countervails ionic imbalance and suggests that it may serve as a potential agent in alleviating neurotoxic effects of β-amyloid peptide.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalSkin Pharmacology and Physiology
Volume17
Issue number4
DOIs
Publication statusPublished - Aug 4 2004

Keywords

  • Alzheimer's disease
  • Calcium
  • Fibroblast
  • Fluorescence
  • Haloperidol
  • Schizophrenia
  • β-amyloid peptide

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Dermatology

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