Haemodiafiltration elicits less platelet activation compared to haemodialysis

Gergely Becs, Renáta Hudák, Zsolt Fejes, Ildikó Beke Debreceni, Harjit Pal Bhattoa, József Balla, János Kappelmayer

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2 Citations (Scopus)

Abstract

Background: Mortality in patients with end-stage renal disorders is often a consequence of cardiovascular complications. Renal replacement therapies may contribute to this morbidity by promoting cellular activation. In renal failure patients peripheral blood samples were investigated for platelet and endothelial cell activation markers to compare the effects of haemodiafiltration (HDF) and haemodialysis (HD). Methods: Overall 28 patients were included in the study. Platelet P-selectin and leukocyte - platelet heterotypic aggregates were studied by flow cytometry. Soluble P- and E-selectin values were determined by ELISA, while von Willebrand factor (vWF) antigen levels were measured by immunoturbidimetry. Statistical analysis was done by the SPSS v22 software. Results: Platelet surface P-selectin was below 3.0 % in healthy controls, but it was higher during the dialysis after 4 h, 8 % and 14.3 % in HDF and HD, respectively. Monocyte-platelet heterotypic aggregates were significantly elevated after 4 h in both treatments, up to 69.2 % in HDF and to 82.9 % in HD. Soluble P-selectin levels were also significantly elevated by the end of both treatment procedures (p < 0.001), vWF antigen values, however, showed elevation only during HD treatment. Conclusions: The attenuated platelet activating effects of HDF compared to HD may contribute to a less unfavourable vascular effect in this treatment modality.

Original languageEnglish
Article number147
JournalBMC Nephrology
Volume17
Issue number1
DOIs
Publication statusPublished - Oct 13 2016

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Keywords

  • ESRD
  • Haemodiafiltration
  • Haemodialysis
  • Monocyte-platelet aggregate
  • P-selectin

ASJC Scopus subject areas

  • Nephrology

Cite this

Becs, G., Hudák, R., Fejes, Z., Debreceni, I. B., Bhattoa, H. P., Balla, J., & Kappelmayer, J. (2016). Haemodiafiltration elicits less platelet activation compared to haemodialysis. BMC Nephrology, 17(1), [147]. https://doi.org/10.1186/s12882-016-0364-x