Gut region-specific rearrangement of the cellular and subcellular compartments of nitric oxide synthase isoforms after chronic ethanol consumption in rats

Nikolett Bódi, Zsanett Jancsó, Petra Talapka, Alexandra Pál, Marietta Zita Poles, Mária Bagyánszki, E. Hermesz, Éva Fekete

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We recently provided evidence of cell-type-specific differences in the subcellular distributions of the three nitric oxide synthase (NOS) isoforms in the myenteric neurons, enteric smooth muscle cells and the capillary endothelium of the rat duodenum. We hypothesized that the presence of three NOS isoforms in the same type of cells with differences in subcellular compartmentalization might reflect a functional plasticity. Therefore, investigation of the possible rearrangement of cellular and subcellular NOS compartments in different gut segments following chronic ethanol treatment was the aim of this study.

Rats were randomly divided into two groups and received water or 20% ethanol solution, preceded by short periods of adaptation with 10% and 15% ethanol. After 8 weeks, segments of duodenum, ileum and colon of the control and the alcohol-treated rats were processed for post-embedding immunohistochemistry and RT-PCR.

The quantitative differences in the numbers of gold particles indicative of the different NOSs and their relative mRNA levels between the two experimental groups varied greatly, depending on the gut segment, and also on the cellular and subcellular compartments investigated. The chronic ethanol administration had the opposite effect on the quantitative distribution of the neuronal and endothelial NOS labelling gold particles in the different cellular compartments and resulted in subcellular rearrangement of NOS labels along the gastrointestinal tract.

The intestinal region-specific rearrangement of the cellular and subcellular NOS compartments may possibly result in functional plasticity and help to maintain the optimum NO level under pathological conditions.

Original languageEnglish
Pages (from-to)1547-1555
Number of pages9
JournalHistology and Histopathology
Volume29
Issue number12
Publication statusPublished - Dec 1 2014

Fingerprint

Nitric Oxide Synthase
Protein Isoforms
Ethanol
Duodenum
Gold
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Vascular Endothelium
Ileum
Smooth Muscle Myocytes
Gastrointestinal Tract
Colon
Immunohistochemistry
Alcohols
Neurons
Polymerase Chain Reaction
Messenger RNA
Water
Therapeutics

Keywords

  • Cellular localization
  • Chronic ethanol consumption
  • Gut region specificity
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Bódi, N., Jancsó, Z., Talapka, P., Pál, A., Poles, M. Z., Bagyánszki, M., ... Fekete, É. (2014). Gut region-specific rearrangement of the cellular and subcellular compartments of nitric oxide synthase isoforms after chronic ethanol consumption in rats. Histology and Histopathology, 29(12), 1547-1555.

Gut region-specific rearrangement of the cellular and subcellular compartments of nitric oxide synthase isoforms after chronic ethanol consumption in rats. / Bódi, Nikolett; Jancsó, Zsanett; Talapka, Petra; Pál, Alexandra; Poles, Marietta Zita; Bagyánszki, Mária; Hermesz, E.; Fekete, Éva.

In: Histology and Histopathology, Vol. 29, No. 12, 01.12.2014, p. 1547-1555.

Research output: Contribution to journalArticle

Bódi, Nikolett ; Jancsó, Zsanett ; Talapka, Petra ; Pál, Alexandra ; Poles, Marietta Zita ; Bagyánszki, Mária ; Hermesz, E. ; Fekete, Éva. / Gut region-specific rearrangement of the cellular and subcellular compartments of nitric oxide synthase isoforms after chronic ethanol consumption in rats. In: Histology and Histopathology. 2014 ; Vol. 29, No. 12. pp. 1547-1555.
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