Guinea pigs immunized with xenogeneic cells

skin test, in vitro stimulation, migration, cytotoxicity of lymph node cells

G. G. Petranyi, E. Klein, A. J. Cochran, E. Svedmyr, H. Jacobson

Research output: Contribution to journalArticle

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Abstract

The lymphocyte response in 3 in vitro tests: blast transformation, inhibition of migration and cytotoxicity was compared with the cutaneous test in the early period of delayed type hypersensitivity. Guinea pigs were immunized with cells of the human lymphoid line in Freund's adjuvant. Non sensitized lymph node cells did not react with the xenogeneic cells in either test. High thymidine incorporation of the immune lymphocyte population and a relatively faster migration without added antigen in vitro was recorded with maximum values on day 6. In the presence of the immunizing cells all in vitro tests were negative 2 days after immunization; maximum activity was found at day 6. These responses paralleled the degree of positivity of the skin test. In spite of the fact that skin test and lymphocyte cytotoxicity could be evoked, the mixed lymphocyte target cell test was negative with mouse cells used as antigen (YCAB) or target cells (A9).

Original languageEnglish
Pages (from-to)319-330
Number of pages12
JournalActa Medica Academiae Scientiarum Hungaricae
Volume30
Issue number4
Publication statusPublished - 1973

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Skin Tests
Guinea Pigs
Lymph Nodes
Lymphocytes
Antigens
Freund's Adjuvant
Delayed Hypersensitivity
Lymphocyte Activation
Thymidine
In Vitro Techniques
Immunization
Population

ASJC Scopus subject areas

  • Medicine(all)

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Guinea pigs immunized with xenogeneic cells : skin test, in vitro stimulation, migration, cytotoxicity of lymph node cells. / Petranyi, G. G.; Klein, E.; Cochran, A. J.; Svedmyr, E.; Jacobson, H.

In: Acta Medica Academiae Scientiarum Hungaricae, Vol. 30, No. 4, 1973, p. 319-330.

Research output: Contribution to journalArticle

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