Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources

Philipp Stockmann, Jung Park, Cornelius Von Wilmowsky, Emeka Nkenke, E. Felszeghy, Jan Friedrich Dehner, Christian Schmitt, Christian Tudor, Karl Andreas Schlegel

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. This animal study was conducted to compare the in vivo osteogenic potential of adipose-derived (AD), periosteum-derived (PD) and bone marrow-derived (BM) mesenchymal stem/progenitor cells (MSC). Autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic differentiation following in vitro cell expansion. Ex vivo cultivated cells were seeded on a collagen scaffold and subsequently added to freshly created monocortical calvarial bone defects in 21 domestic pigs. Pure collagen scaffold served as a control defect. The animals were sacrificed at specific time points and de novo bone formation was quantitatively analyzed by histomorphometry. Bone volume/total defect volume (BV/TV) and the mineralization rate of newly formed bone were compared among the groups. In the early stages of wound healing, up to 30 days, the test defects did not show better bone regeneration than those in the control defect, but the bone healing process in the test defects was accelerated in the later stage compared to those in the control defect. All the test defects showed complete osseous healing after 90 days compared to those in the control defect. During the observation period, no significant differences in BV/TV and mineralization of newly formed bone among the test defects were observed. Irrespective of the tissue sources of MSC, the speed and pattern of osseous healing after cell transplantations into monocortical bone defects were comparable. Our results indicate that the efficiency of autologous AD-MSC, PD-MSC and BM-MSC transplantation following ex vivo cell expansion is not significantly different for the guided regeneration of bone defects.

Original languageEnglish
Pages (from-to)310-320
Number of pages11
JournalJournal of Cranio-Maxillofacial Surgery
Volume40
Issue number4
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Bone Regeneration
Mesenchymal Stromal Cells
Swine
Bone and Bones
Periosteum
Collagen
Bone Marrow
Mesenchymal Stem Cell Transplantation
Bone Substitutes
Sus scrofa
Bone Transplantation
Cell Transplantation
Tissue Engineering
Osteogenesis
Wound Healing
Observation
Morbidity

Keywords

  • Adipose tissue
  • Bone substitute
  • Guided bone regeneration
  • Mesenchymal stem/progenitor cells
  • Pig model

ASJC Scopus subject areas

  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources. / Stockmann, Philipp; Park, Jung; Von Wilmowsky, Cornelius; Nkenke, Emeka; Felszeghy, E.; Dehner, Jan Friedrich; Schmitt, Christian; Tudor, Christian; Schlegel, Karl Andreas.

In: Journal of Cranio-Maxillofacial Surgery, Vol. 40, No. 4, 06.2012, p. 310-320.

Research output: Contribution to journalArticle

Stockmann, Philipp ; Park, Jung ; Von Wilmowsky, Cornelius ; Nkenke, Emeka ; Felszeghy, E. ; Dehner, Jan Friedrich ; Schmitt, Christian ; Tudor, Christian ; Schlegel, Karl Andreas. / Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources. In: Journal of Cranio-Maxillofacial Surgery. 2012 ; Vol. 40, No. 4. pp. 310-320.
@article{25fbb237c5fb4a538c599f2ca71ec34f,
title = "Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources",
abstract = "Due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. This animal study was conducted to compare the in vivo osteogenic potential of adipose-derived (AD), periosteum-derived (PD) and bone marrow-derived (BM) mesenchymal stem/progenitor cells (MSC). Autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic differentiation following in vitro cell expansion. Ex vivo cultivated cells were seeded on a collagen scaffold and subsequently added to freshly created monocortical calvarial bone defects in 21 domestic pigs. Pure collagen scaffold served as a control defect. The animals were sacrificed at specific time points and de novo bone formation was quantitatively analyzed by histomorphometry. Bone volume/total defect volume (BV/TV) and the mineralization rate of newly formed bone were compared among the groups. In the early stages of wound healing, up to 30 days, the test defects did not show better bone regeneration than those in the control defect, but the bone healing process in the test defects was accelerated in the later stage compared to those in the control defect. All the test defects showed complete osseous healing after 90 days compared to those in the control defect. During the observation period, no significant differences in BV/TV and mineralization of newly formed bone among the test defects were observed. Irrespective of the tissue sources of MSC, the speed and pattern of osseous healing after cell transplantations into monocortical bone defects were comparable. Our results indicate that the efficiency of autologous AD-MSC, PD-MSC and BM-MSC transplantation following ex vivo cell expansion is not significantly different for the guided regeneration of bone defects.",
keywords = "Adipose tissue, Bone substitute, Guided bone regeneration, Mesenchymal stem/progenitor cells, Pig model",
author = "Philipp Stockmann and Jung Park and {Von Wilmowsky}, Cornelius and Emeka Nkenke and E. Felszeghy and Dehner, {Jan Friedrich} and Christian Schmitt and Christian Tudor and Schlegel, {Karl Andreas}",
year = "2012",
month = "6",
doi = "10.1016/j.jcms.2011.05.004",
language = "English",
volume = "40",
pages = "310--320",
journal = "Journal of Cranio-Maxillo-Facial Surgery",
issn = "1010-5182",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - A comparison of different tissue sources

AU - Stockmann, Philipp

AU - Park, Jung

AU - Von Wilmowsky, Cornelius

AU - Nkenke, Emeka

AU - Felszeghy, E.

AU - Dehner, Jan Friedrich

AU - Schmitt, Christian

AU - Tudor, Christian

AU - Schlegel, Karl Andreas

PY - 2012/6

Y1 - 2012/6

N2 - Due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. This animal study was conducted to compare the in vivo osteogenic potential of adipose-derived (AD), periosteum-derived (PD) and bone marrow-derived (BM) mesenchymal stem/progenitor cells (MSC). Autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic differentiation following in vitro cell expansion. Ex vivo cultivated cells were seeded on a collagen scaffold and subsequently added to freshly created monocortical calvarial bone defects in 21 domestic pigs. Pure collagen scaffold served as a control defect. The animals were sacrificed at specific time points and de novo bone formation was quantitatively analyzed by histomorphometry. Bone volume/total defect volume (BV/TV) and the mineralization rate of newly formed bone were compared among the groups. In the early stages of wound healing, up to 30 days, the test defects did not show better bone regeneration than those in the control defect, but the bone healing process in the test defects was accelerated in the later stage compared to those in the control defect. All the test defects showed complete osseous healing after 90 days compared to those in the control defect. During the observation period, no significant differences in BV/TV and mineralization of newly formed bone among the test defects were observed. Irrespective of the tissue sources of MSC, the speed and pattern of osseous healing after cell transplantations into monocortical bone defects were comparable. Our results indicate that the efficiency of autologous AD-MSC, PD-MSC and BM-MSC transplantation following ex vivo cell expansion is not significantly different for the guided regeneration of bone defects.

AB - Due to donor side morbidity and the absence of osteogenic properties in bone substitutes, there is a growing need for an alternative to traditional bone grafting within the scope of tissue engineering. This animal study was conducted to compare the in vivo osteogenic potential of adipose-derived (AD), periosteum-derived (PD) and bone marrow-derived (BM) mesenchymal stem/progenitor cells (MSC). Autologous mesenchymal stem/progenitor cells of named tissue origin were induced into osteogenic differentiation following in vitro cell expansion. Ex vivo cultivated cells were seeded on a collagen scaffold and subsequently added to freshly created monocortical calvarial bone defects in 21 domestic pigs. Pure collagen scaffold served as a control defect. The animals were sacrificed at specific time points and de novo bone formation was quantitatively analyzed by histomorphometry. Bone volume/total defect volume (BV/TV) and the mineralization rate of newly formed bone were compared among the groups. In the early stages of wound healing, up to 30 days, the test defects did not show better bone regeneration than those in the control defect, but the bone healing process in the test defects was accelerated in the later stage compared to those in the control defect. All the test defects showed complete osseous healing after 90 days compared to those in the control defect. During the observation period, no significant differences in BV/TV and mineralization of newly formed bone among the test defects were observed. Irrespective of the tissue sources of MSC, the speed and pattern of osseous healing after cell transplantations into monocortical bone defects were comparable. Our results indicate that the efficiency of autologous AD-MSC, PD-MSC and BM-MSC transplantation following ex vivo cell expansion is not significantly different for the guided regeneration of bone defects.

KW - Adipose tissue

KW - Bone substitute

KW - Guided bone regeneration

KW - Mesenchymal stem/progenitor cells

KW - Pig model

UR - http://www.scopus.com/inward/record.url?scp=84860675226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860675226&partnerID=8YFLogxK

U2 - 10.1016/j.jcms.2011.05.004

DO - 10.1016/j.jcms.2011.05.004

M3 - Article

VL - 40

SP - 310

EP - 320

JO - Journal of Cranio-Maxillo-Facial Surgery

JF - Journal of Cranio-Maxillo-Facial Surgery

SN - 1010-5182

IS - 4

ER -