Guanine-cytosine rich regions of plasmid DNA can be the target in anti-plasmid effect of phenothiazines

Csilla Miskolci, Imre Labádi, Teruo Kurihara, Noboru Motohashi, Joseph Molnár

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The antiplasmid effects of promethazine on E. coli is the consequence of special complex formed with a covalently closed circular (ccc) form of plasmid DNA. The exact target in this macromolecule, however, was not clarified until recently. Caffeine and the chemically similar guanosine-5'- monophosphate (GMP) could compete with the antiplasmid effect of promethazine, showing that promethazine or other phenothiazines preferentially bind to xanthine type molecules. Among the xanthines, GMP was more effective at complex-forming than adenosine-5'-monophosphate (AMP). In addition, the Z-DNA was more susceptible than B-DNA. Therefore, one could suppose that guanine-cytosine (G-C) rich regions have higher affinity than adenine-thymine (A-T) rich region on phenothiazines. Because the G-C rich regions have a special role in the DNA stability via three hydrogen bonds, we suppose that these regions could have a key role in some biological effects such as antiplasmid and anticancer activity. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalInternational Journal of Antimicrobial Agents
Volume14
Issue number3
DOIs
Publication statusPublished - Apr 1 2000

Keywords

  • Caffeine
  • Guanosine monophosphate (GMP)
  • Plasmid
  • Promethazine

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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