GTPase activity of small GTP-binding proteins in HL-60 membranes is stimulated by arachidonic acid

E. Ligeti, V. Pizon, A. Wittinghofer, P. Gierschik, K. H. Jakobs

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Abstract

The GTPase activity of membranes isolated from differentiated HL-60 cells was investigated to obtain information about the possible involvement of membrane-bound GTP-binding proteins in the regulation of the NADPH oxidase. A more than tenfold increase in the rate of hydrolysis of membrane-bound GTP was observed when cytosol and arachidonic acid were added simultanously, i.e. under the same conditions where NADPH oxidase becomes activated. There were parallel changes in GTPase and NADPH oxidase activities when the concentration of arachidonic acid or the species of the fatty acid was varied or different detergents were applied. Separation of the GTP-binding proteins of the solubilized membrane by sucrose density gradient centrifugation, allowed us to ascribe the observed effect to the stimulation of the GTPase activity of small GTP-binding proteins by cytosolic component(s). Indirect evidence suggests that, in contrast to the effect upon recombinant ras and ras-GTPase-activating protein, in intact HL-60 membranes the interaction of rap1A with rap-GTPase-activating protein, is strongly enhanced by arachidonic acid.

Original languageEnglish
Pages (from-to)813-820
Number of pages8
JournalEuropean Journal of Biochemistry
Volume216
Issue number3
Publication statusPublished - 1993

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Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
GTP-Binding Proteins
Arachidonic Acid
NADPH Oxidase
Membranes
ras GTPase-Activating Proteins
GTPase-Activating Proteins
Density Gradient Centrifugation
Centrifugation
HL-60 Cells
Guanosine Triphosphate
Detergents
Cytosol
Sucrose
Hydrolysis
Fatty Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

GTPase activity of small GTP-binding proteins in HL-60 membranes is stimulated by arachidonic acid. / Ligeti, E.; Pizon, V.; Wittinghofer, A.; Gierschik, P.; Jakobs, K. H.

In: European Journal of Biochemistry, Vol. 216, No. 3, 1993, p. 813-820.

Research output: Contribution to journalArticle

Ligeti, E, Pizon, V, Wittinghofer, A, Gierschik, P & Jakobs, KH 1993, 'GTPase activity of small GTP-binding proteins in HL-60 membranes is stimulated by arachidonic acid', European Journal of Biochemistry, vol. 216, no. 3, pp. 813-820.
Ligeti, E. ; Pizon, V. ; Wittinghofer, A. ; Gierschik, P. ; Jakobs, K. H. / GTPase activity of small GTP-binding proteins in HL-60 membranes is stimulated by arachidonic acid. In: European Journal of Biochemistry. 1993 ; Vol. 216, No. 3. pp. 813-820.
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