Growth conditions for the expression of fibronectin, collagen type I, vitronectin, and laminin binding to Escherichia coli strain NG7C

A. Ljungh, L. Emödy, P. Aleljung, O. Olusanya, T. Wadström

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17 Citations (Scopus)

Abstract

The ability of Escherichia coli strains isolated from various human infections to bind fibronectin (Fn), collagen type I (Cn), laminin (Ln), and vitronectin (Vn) was studied. Binding of the proteins was shown to be specific and to be inhibited after protease treatment and heating of bacterial cells at 80°C. Binding of Fn, Cn type I, and Ln was not expressed in CFA broth and Voccani's medium with sodium chloride concentration above 0.5%. Fn binding was specifically enhanced for cells grown in CFA broth and Voccani's medium containing CaCl2 (up to 10 m M final concentration), whereas binding of Cn type I was decreased. Growth in liquid medium yielded cells with maximal binding of Fn, Cn type I, Vn, and Ln after 20-22 h of growth. The binding of Fn, Cn type I, and Vn to cells of strain NG7C was inhibited for cells preincubated with antisera raised to the homologous strain;E. coli NG7C cells seem to have specific binding sites for Fn, Cn type I, Vn, and possibly also Ln.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalCurrent Microbiology
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 1 1991

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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