Growth and colonization suppression of Salmonella enterica serovar Hadar in vitro and in vivo

Noémi Nógrády, Ariel Imre, Ivan Rychlik, Paul A. Barrow, Béla Nagy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Growth suppression in Salmonella enterica serovar Hadar (S. Hadar) was investigated, in vitro under strict anaerobiosis and in vivo in the intestine of the day-old chicken. Stationary-phase cultures of 20 S. Hadar field strains were tested against each other for growth suppression activity by their ability to suppress the multiplication of low counts of minority cultures inoculated into them as nalidixic acid-resistant mutants. All strains showed profound growth suppression. Four S. Hadar strains were selected and further tested for their ability to suppress growth of S. Enteritidis, S. Typhimurium, S. Virchow and S. Saintpaul. One of the four strains (S. Hadar 18) was randomly selected for further studies. Precolonization of chicken with S. Hadar 18 prevented superinfection with any of the serovars mentioned above. From more than 1000 TnphoA mutants of S. Hadar 18 screened against the parent strain anaerobically in vitro, four were non-suppressive with TnphoA insertions in dapF, aroD, sgaT or tatA. Only the dapF mutant was also non-suppressive in the chicken intestine.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalFEMS microbiology letters
Volume218
Issue number1
DOIs
Publication statusPublished - Jan 21 2003

Keywords

  • Growth suppression
  • Intestinal colonization
  • Salmonella Hadar
  • Stationary phase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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