Green tea flavonols inhibit glucosidase II

Alessandra Gamberucci, Laura Konta, Angela Colucci, Roberta Giunti, Judit É Magyar, J. Mandl, G. Bánhegyi, Angelo Benedetti, M. Csala

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Green tea is getting into the focus of scientific interest due to its beneficial health effects, most of which are attributed to its catechin content. Polyphenolic tea catechins have antioxidant, antiproliferative, antiangiogenic and proapoptotic effects, which makes them promising anticancer compounds. Other poly-hydroxy molecules have similar antitumor potentials through the inhibition of glucosidase II, which affects the glycoprotein maturation and quality control in the endoplasmic reticulum. We investigated the effect of tea catechins on glucosidase II activity in rat liver microsomes using 4-methylumbelliferyl glucoside and 4-nitrophenyl glucoside as substrates. A concentration-dependent inhibition with non-competitive kinetics was found. The IC50 and Ki values for certain tea catechins were comparable with those of N-butyldeoxynojirimycin, the widely used glucosidase inhibitor. The possible interference of tea catechins with the glycoprotein processing in the endoplasmic reticulum should be considered as a potential mechanism of their dietary or pharmacological effects.

Original languageEnglish
Pages (from-to)640-646
Number of pages7
JournalBiochemical Pharmacology
Volume72
Issue number5
DOIs
Publication statusPublished - Aug 28 2006

Fingerprint

Flavonols
Catechin
Tea
Endoplasmic Reticulum
Glycoproteins
Glucosidases
Glucosides
Liver Microsomes
Quality Control
Liver
Inhibitory Concentration 50
Quality control
Rats
Antioxidants
4-nitrophenyl-alpha-glucosidase
Health
Pharmacology
Molecules
Kinetics
Substrates

Keywords

  • Endoplasmic reticulum
  • Glucosidase II
  • Green tea
  • Inhibition
  • Kinetics
  • Rat liver

ASJC Scopus subject areas

  • Pharmacology

Cite this

Gamberucci, A., Konta, L., Colucci, A., Giunti, R., Magyar, J. É., Mandl, J., ... Csala, M. (2006). Green tea flavonols inhibit glucosidase II. Biochemical Pharmacology, 72(5), 640-646. https://doi.org/10.1016/j.bcp.2006.05.016

Green tea flavonols inhibit glucosidase II. / Gamberucci, Alessandra; Konta, Laura; Colucci, Angela; Giunti, Roberta; Magyar, Judit É; Mandl, J.; Bánhegyi, G.; Benedetti, Angelo; Csala, M.

In: Biochemical Pharmacology, Vol. 72, No. 5, 28.08.2006, p. 640-646.

Research output: Contribution to journalArticle

Gamberucci, A, Konta, L, Colucci, A, Giunti, R, Magyar, JÉ, Mandl, J, Bánhegyi, G, Benedetti, A & Csala, M 2006, 'Green tea flavonols inhibit glucosidase II', Biochemical Pharmacology, vol. 72, no. 5, pp. 640-646. https://doi.org/10.1016/j.bcp.2006.05.016
Gamberucci A, Konta L, Colucci A, Giunti R, Magyar JÉ, Mandl J et al. Green tea flavonols inhibit glucosidase II. Biochemical Pharmacology. 2006 Aug 28;72(5):640-646. https://doi.org/10.1016/j.bcp.2006.05.016
Gamberucci, Alessandra ; Konta, Laura ; Colucci, Angela ; Giunti, Roberta ; Magyar, Judit É ; Mandl, J. ; Bánhegyi, G. ; Benedetti, Angelo ; Csala, M. / Green tea flavonols inhibit glucosidase II. In: Biochemical Pharmacology. 2006 ; Vol. 72, No. 5. pp. 640-646.
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