Graves' IgG recognizes linear epitopes in the human thyrotropin receptor

E. Nagy, Henry B. Burch, Karen Mahoney, Yvonne G. Lukes, John C. Morris, Kenneth D. Burman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Twenty-nine peptides covering the full extracellular domain of the human thyrotropin receptor have been synthesized and tested for reactivity with Graves' patients' and normal sera in ELISA. Two peptides, amino acids 331-350 and the second extracellular loop of the transmembrane segment, bound IgG-s from 5 and 4 of 10 Graves' disease patients' sera, respectively. Neither of these two peptides showed enhanced binding to normal IgG. There were no apparent differences between the Graves' disease and normal group with respect to the other 27 peptides. We conclude that peptide 331-350 and the second extracellular loop carry important linear epitopes which may contribute to the disease process in selected Graves' patients.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number1
DOIs
Publication statusPublished - Oct 15 1992

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Thyrotropin Receptors
Epitopes
Immunoglobulin G
Peptides
Graves Disease
Serum
Enzyme-Linked Immunosorbent Assay
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Graves' IgG recognizes linear epitopes in the human thyrotropin receptor. / Nagy, E.; Burch, Henry B.; Mahoney, Karen; Lukes, Yvonne G.; Morris, John C.; Burman, Kenneth D.

In: Biochemical and Biophysical Research Communications, Vol. 188, No. 1, 15.10.1992, p. 28-33.

Research output: Contribution to journalArticle

Nagy, E. ; Burch, Henry B. ; Mahoney, Karen ; Lukes, Yvonne G. ; Morris, John C. ; Burman, Kenneth D. / Graves' IgG recognizes linear epitopes in the human thyrotropin receptor. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 188, No. 1. pp. 28-33.
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