Grapefruit seed extract inhibits the formation of amyloid-like fibrils by trypsin in aqueous ethanol

Phanindra Babu Kasi, Attila Borics, Mónika Varga, Gábor Endre, Kinga Molnár, L. László, M. Kotormán

Research output: Contribution to journalArticle

2 Citations (Scopus)


Several natural compounds deriving from plants are known to be efficient anti-amyloid aggregation agents. In this study, anti-aggregation activity of grapefruit seed extract was investigated using trypsin as a model protein in aqueous ethanol at pH 7.0. Using turbidity measurement, Congo red (CR) binding assay, electronic circular dichroism (ECD) and transmission electron microscopy (TEM), we found that grapefruit seed extract has ability to inhibit trypsin amyloidlike fibril formation in vitro, and effectiveness increases with growing concentration of grapefruit seed extract. The total phenolic content of it was determined. The results showed that in addition to the polyphenolic compounds some other compounds are also responsible for the fibril formation inhibitory effect. We indicated it for the first time that limonin has anti-fibrillation effect.

Original languageEnglish
Pages (from-to)1437-1440
Number of pages4
JournalNatural Product Communications
Issue number11
Publication statusPublished - Jan 1 2018


  • Amyloid-like fibrils
  • Congo red
  • Grapefruit seed extract
  • Inhibitory effect
  • Limonin
  • Polyphenols
  • Trypsin

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Plant Science
  • Complementary and alternative medicine

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