Granule cells are the main source of excitatory input to a subpopulation of GABAergic hippocampal neurons as revealed by electron microscopic double staining for zinc histochemistry and parvalbumin immunocytochemistry

L. Séress, H. Ábrahám, M. Paleszter, F. Gallyas

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24 Citations (Scopus)

Abstract

Immunocytochemistry was combined with a recent modification of Timm's method to evaluate semi-quantitatively the mossy fiber innervation of dendrites and somata of parvalbumin-containing neurons of the hilus of the dentate gyrus and the CA3 area of Ammon's horn. Using this electron microscopic double staining technique, it was found that (1) the overwhelming majority (95%) of terminals forming asymmetric synapses with parvalbumin-positive dendrites in the dentate hilus, and the strata pyramidale and lucidum of the CA3 area of Ammon's horn, originated from granule cells; (2) two-thirds of the asymmetric axosomatic terminals of parvalbumin-positive neurons contained zinc; and (3) no zinc-containing axon terminals formed synapses with somata or main dendritic shafts of the granule cells.

Original languageEnglish
Pages (from-to)456-462
Number of pages7
JournalExperimental Brain Research
Volume137
Issue number1
Publication statusPublished - 2001

Fingerprint

GABAergic Neurons
Parvalbumins
Hippocampal CA3 Region
Zinc
Immunohistochemistry
Carisoprodol
Electrons
Staining and Labeling
Dendrites
Synapses
Hippocampus
Neurons
Dentate Gyrus
Presynaptic Terminals

Keywords

  • Hippocampus
  • Local-circuit neurons
  • Mossy fiber terminals
  • Synaptic circuitry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Immunocytochemistry was combined with a recent modification of Timm's method to evaluate semi-quantitatively the mossy fiber innervation of dendrites and somata of parvalbumin-containing neurons of the hilus of the dentate gyrus and the CA3 area of Ammon's horn. Using this electron microscopic double staining technique, it was found that (1) the overwhelming majority (95{\%}) of terminals forming asymmetric synapses with parvalbumin-positive dendrites in the dentate hilus, and the strata pyramidale and lucidum of the CA3 area of Ammon's horn, originated from granule cells; (2) two-thirds of the asymmetric axosomatic terminals of parvalbumin-positive neurons contained zinc; and (3) no zinc-containing axon terminals formed synapses with somata or main dendritic shafts of the granule cells.",
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T1 - Granule cells are the main source of excitatory input to a subpopulation of GABAergic hippocampal neurons as revealed by electron microscopic double staining for zinc histochemistry and parvalbumin immunocytochemistry

AU - Séress, L.

AU - Ábrahám, H.

AU - Paleszter, M.

AU - Gallyas, F.

PY - 2001

Y1 - 2001

N2 - Immunocytochemistry was combined with a recent modification of Timm's method to evaluate semi-quantitatively the mossy fiber innervation of dendrites and somata of parvalbumin-containing neurons of the hilus of the dentate gyrus and the CA3 area of Ammon's horn. Using this electron microscopic double staining technique, it was found that (1) the overwhelming majority (95%) of terminals forming asymmetric synapses with parvalbumin-positive dendrites in the dentate hilus, and the strata pyramidale and lucidum of the CA3 area of Ammon's horn, originated from granule cells; (2) two-thirds of the asymmetric axosomatic terminals of parvalbumin-positive neurons contained zinc; and (3) no zinc-containing axon terminals formed synapses with somata or main dendritic shafts of the granule cells.

AB - Immunocytochemistry was combined with a recent modification of Timm's method to evaluate semi-quantitatively the mossy fiber innervation of dendrites and somata of parvalbumin-containing neurons of the hilus of the dentate gyrus and the CA3 area of Ammon's horn. Using this electron microscopic double staining technique, it was found that (1) the overwhelming majority (95%) of terminals forming asymmetric synapses with parvalbumin-positive dendrites in the dentate hilus, and the strata pyramidale and lucidum of the CA3 area of Ammon's horn, originated from granule cells; (2) two-thirds of the asymmetric axosomatic terminals of parvalbumin-positive neurons contained zinc; and (3) no zinc-containing axon terminals formed synapses with somata or main dendritic shafts of the granule cells.

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KW - Synaptic circuitry

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