Gradient HPLC separation of dehydroepiandrosterone (DHEA) from its metabolites and biological congeners: Role of tetrahydrofuran in the chromatographic mechanism

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A three-step gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the separation of dehydroepiandrosterone (DHEA), its sulfate ester (DHEA-S), its three C7-oxidized metabolites (7αOH-DHEA, 7βOH-DHEA, 7-keto-DHEA), and its biosynthetic congeners (androstenedione, testosterone, estradiol, pregnenolone). This new method allows the quantitative characterization of DHEA metabolism and biosynthetic transformation under given physiological, pathological, or therapeutically influenced circumstances. Tetrahydrofuran probably acts as a proton acceptor coadsorbent, while isopropanol behaves as a proton donor during the separation of testosterone, estradiol, and the stereoisomers of 7-OH-DHEA. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)2105-2109
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Issue number8
Publication statusPublished - Aug 1 2009



  • 7-DHEA derivatives
  • DHEA biosynthetic congeners
  • DHEA metabolites
  • Dehydroepiandrosterone (DHEA)
  • Gradient RP-HPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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