Gonadotropin secretion and inositol phosphate production: influence of lithium chloride in vitro

T. A. Karenberg, L. Kiesel, T. Rabe, K. Klinga, T. Von Holst, A. Spat, B. Runnebaum

Research output: Contribution to journalArticle

Abstract

Polyphosphoinositide breakdown to inositol phosphates is an important postreceptor mechanism in various ligand-stimulated cell types. LiCl is a commonly used tool to study inositol phosphate accumulation since it inhibits inositol phosphate metabolism and is a widely distributed drug in neurology. In the present study the conditions of LiCl application have been characterized in rat pituitary cells to allow further investigations of GnHR-induced gonadotropin release regarding the involvement of inositol phosphate production. In rat anterior pituitary cells radiolabelled with myo-(2-3H)-inositol, LiCl (10 mM) enhances the amount of intracellular inositol phosphates (IP) in stimulated and unstimulated cells. The production rates of inositol monophosphate (IP1) and inositol biphosphate (IP2) are maximally stimulated at a different concentration of LiCl (1 mM 890% and 10 mM 493% resp.). Preincubation times shorter than 30 min with 10 mM LiCl provide the highest production rate concerning IP1 (2.7 fold) and 2 h the highest production rate concerning IP2 (10.2 fold). GnRH-induced LH release over 4 h is stimulated by preincubation with LiCl for 10 min and this provides evidence for a role of inositol phosphate production in the mechanism of GnRH. In addition, the methods developed in this paper allow the determination of inositol phosphates under more accurately defined conditions.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalAktuelle Endokrinologie und Stoffwechsel
Volume9
Issue number3
Publication statusPublished - Jan 1 1988

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Karenberg, T. A., Kiesel, L., Rabe, T., Klinga, K., Von Holst, T., Spat, A., & Runnebaum, B. (1988). Gonadotropin secretion and inositol phosphate production: influence of lithium chloride in vitro. Aktuelle Endokrinologie und Stoffwechsel, 9(3), 161-167.