Gold nanohybrid systems with tunable fluorescent feature: Interaction of cysteine and cysteine-containing peptides with gold in two- and three-dimensional systems

Edit Csapó, Ditta Ungor, Ádám Juhász, G. Tóth, I. Dékány

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interactions of L-cysteine (Cys), L-cysteinyl-tryptophan (Cys-Trp) and L-glutathione (γ-Glu-Cys-Gly, GSH) with gold-coated surface (Au0) and tetrachloroaurate ions (AuCl4 ) were studied in two(2D)- and three(3D)-dimensional systems, respectively. The irreversible bindings of the molecules onto gold surface via gold-sulphur (Au-S) bonds were proven by 2D quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. Moreover, the cross-sectional areas and the possible orientation of these biomolecules on gold film were also determined and compared with the theoretical calculated values. The spontaneous interactions of Cys, Cys-Trp and GSH with AuCl4 were studied in aqueous medium at 37 °C as well. Depending on the pH and the molar ratios of AuCl4 /molecules, the formation of diverse gold nanostructures were confirmed. It was proven that the spontaneous interactions of AuCl4 with Cys and GSH result in the formation of orange- and yellow-emitting gold nanohybrid systems (λemission = 620 and 590 nm) consist of a self-assembly structure at acidic conditions. In contrast, at alkaline medium fluorescent Au nanostructures were not formed in the AuCl4 /Cys system, but ultra-small blue-emitting gold nanoclusters (Au NCs) were identified in the samples containing AuCl4 /GSH. For AuCl4 /Cys-Trp systems the presence of Trp aromatic side chain prevents the formation of the fluorescence self-assembly nanostructure and depending on the pH, Au concentration and ratio of the reactants the appearance of dipeptide-stabilized plasmonic gold nanoparticles (Au NPs) or supramolecular gold complexes having intense blue fluorescence (λ = 470 nm) were observed.

Original languageEnglish
Pages (from-to)264-271
Number of pages8
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume511
DOIs
Publication statusPublished - Dec 20 2016

Fingerprint

cysteine
Gold
Peptides
peptides
Cysteine
gold
tryptophan
interactions
Tryptophan
Nanostructures
Self assembly
self assembly
Fluorescence
fluorescence
Molecules
glutathione
Dipeptides
Nanoclusters
Quartz crystal microbalances
Surface plasmon resonance

Keywords

  • Gold nanoclusters
  • Gold nanoparticles
  • QCM
  • Self-assembly structure
  • SPR
  • Tunable photoluminescence

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

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title = "Gold nanohybrid systems with tunable fluorescent feature: Interaction of cysteine and cysteine-containing peptides with gold in two- and three-dimensional systems",
abstract = "Interactions of L-cysteine (Cys), L-cysteinyl-tryptophan (Cys-Trp) and L-glutathione (γ-Glu-Cys-Gly, GSH) with gold-coated surface (Au0) and tetrachloroaurate ions (AuCl4 −) were studied in two(2D)- and three(3D)-dimensional systems, respectively. The irreversible bindings of the molecules onto gold surface via gold-sulphur (Au-S) bonds were proven by 2D quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. Moreover, the cross-sectional areas and the possible orientation of these biomolecules on gold film were also determined and compared with the theoretical calculated values. The spontaneous interactions of Cys, Cys-Trp and GSH with AuCl4 − were studied in aqueous medium at 37 °C as well. Depending on the pH and the molar ratios of AuCl4 −/molecules, the formation of diverse gold nanostructures were confirmed. It was proven that the spontaneous interactions of AuCl4 − with Cys and GSH result in the formation of orange- and yellow-emitting gold nanohybrid systems (λemission = 620 and 590 nm) consist of a self-assembly structure at acidic conditions. In contrast, at alkaline medium fluorescent Au nanostructures were not formed in the AuCl4 −/Cys system, but ultra-small blue-emitting gold nanoclusters (Au NCs) were identified in the samples containing AuCl4 −/GSH. For AuCl4 −/Cys-Trp systems the presence of Trp aromatic side chain prevents the formation of the fluorescence self-assembly nanostructure and depending on the pH, Au concentration and ratio of the reactants the appearance of dipeptide-stabilized plasmonic gold nanoparticles (Au NPs) or supramolecular gold complexes having intense blue fluorescence (λ = 470 nm) were observed.",
keywords = "Gold nanoclusters, Gold nanoparticles, QCM, Self-assembly structure, SPR, Tunable photoluminescence",
author = "Edit Csap{\'o} and Ditta Ungor and {\'A}d{\'a}m Juh{\'a}sz and G. T{\'o}th and I. D{\'e}k{\'a}ny",
year = "2016",
month = "12",
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language = "English",
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TY - JOUR

T1 - Gold nanohybrid systems with tunable fluorescent feature

T2 - Interaction of cysteine and cysteine-containing peptides with gold in two- and three-dimensional systems

AU - Csapó, Edit

AU - Ungor, Ditta

AU - Juhász, Ádám

AU - Tóth, G.

AU - Dékány, I.

PY - 2016/12/20

Y1 - 2016/12/20

N2 - Interactions of L-cysteine (Cys), L-cysteinyl-tryptophan (Cys-Trp) and L-glutathione (γ-Glu-Cys-Gly, GSH) with gold-coated surface (Au0) and tetrachloroaurate ions (AuCl4 −) were studied in two(2D)- and three(3D)-dimensional systems, respectively. The irreversible bindings of the molecules onto gold surface via gold-sulphur (Au-S) bonds were proven by 2D quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. Moreover, the cross-sectional areas and the possible orientation of these biomolecules on gold film were also determined and compared with the theoretical calculated values. The spontaneous interactions of Cys, Cys-Trp and GSH with AuCl4 − were studied in aqueous medium at 37 °C as well. Depending on the pH and the molar ratios of AuCl4 −/molecules, the formation of diverse gold nanostructures were confirmed. It was proven that the spontaneous interactions of AuCl4 − with Cys and GSH result in the formation of orange- and yellow-emitting gold nanohybrid systems (λemission = 620 and 590 nm) consist of a self-assembly structure at acidic conditions. In contrast, at alkaline medium fluorescent Au nanostructures were not formed in the AuCl4 −/Cys system, but ultra-small blue-emitting gold nanoclusters (Au NCs) were identified in the samples containing AuCl4 −/GSH. For AuCl4 −/Cys-Trp systems the presence of Trp aromatic side chain prevents the formation of the fluorescence self-assembly nanostructure and depending on the pH, Au concentration and ratio of the reactants the appearance of dipeptide-stabilized plasmonic gold nanoparticles (Au NPs) or supramolecular gold complexes having intense blue fluorescence (λ = 470 nm) were observed.

AB - Interactions of L-cysteine (Cys), L-cysteinyl-tryptophan (Cys-Trp) and L-glutathione (γ-Glu-Cys-Gly, GSH) with gold-coated surface (Au0) and tetrachloroaurate ions (AuCl4 −) were studied in two(2D)- and three(3D)-dimensional systems, respectively. The irreversible bindings of the molecules onto gold surface via gold-sulphur (Au-S) bonds were proven by 2D quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) techniques. Moreover, the cross-sectional areas and the possible orientation of these biomolecules on gold film were also determined and compared with the theoretical calculated values. The spontaneous interactions of Cys, Cys-Trp and GSH with AuCl4 − were studied in aqueous medium at 37 °C as well. Depending on the pH and the molar ratios of AuCl4 −/molecules, the formation of diverse gold nanostructures were confirmed. It was proven that the spontaneous interactions of AuCl4 − with Cys and GSH result in the formation of orange- and yellow-emitting gold nanohybrid systems (λemission = 620 and 590 nm) consist of a self-assembly structure at acidic conditions. In contrast, at alkaline medium fluorescent Au nanostructures were not formed in the AuCl4 −/Cys system, but ultra-small blue-emitting gold nanoclusters (Au NCs) were identified in the samples containing AuCl4 −/GSH. For AuCl4 −/Cys-Trp systems the presence of Trp aromatic side chain prevents the formation of the fluorescence self-assembly nanostructure and depending on the pH, Au concentration and ratio of the reactants the appearance of dipeptide-stabilized plasmonic gold nanoparticles (Au NPs) or supramolecular gold complexes having intense blue fluorescence (λ = 470 nm) were observed.

KW - Gold nanoclusters

KW - Gold nanoparticles

KW - QCM

KW - Self-assembly structure

KW - SPR

KW - Tunable photoluminescence

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U2 - 10.1016/j.colsurfa.2016.10.003

DO - 10.1016/j.colsurfa.2016.10.003

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VL - 511

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JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

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