Glycosylation site analysis of human alpha-1-acid glycoprotein (AGP) by capillary liquid chromatography - Electrospray mass spectrometry

T. Imre, G. Schlosser, Gabriella Pocsfalvi, Rosa Siciliano, Éva Molnár-Szöllosi, T. Kremmer, Antonio Malorni, K. Vékey

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A new anionic surfactant (RapiGest SF) was successfully used for site-specific analysis of glycosylation in human alpha-1-acid glycoprotein (AGP). By means of this analytical approach combined with capillary HPLC-mass spectrometry (and tandem mass spectrometry), the N-linked glycosylation pattern of AGP was explored. On the basis of mass matching and MS/MS experiments ca 80 different AGP-derived glycopeptides were identified. Glycosylation shows a markedly different pattern for the various glycosylation sites. At sites I and II, triantennary complex-type oligosaccharides predominate and at sites III, IV and V, tetra-antennary complex-type oligosaccharides predominate. Sites IV and V show the presence of additional N-acetyl lactosamine (Gal-GlcNAc) units (even higher degree of branching and/or longer antennae are also present).

Original languageEnglish
Pages (from-to)1472-1483
Number of pages12
JournalJournal of Mass Spectrometry
Volume40
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Glycosylation
Orosomucoid
Glycoproteins
Liquid chromatography
liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
mass spectroscopy
Oligosaccharides
acids
Acids
Tandem Mass Spectrometry
Glycopeptides
Anionic surfactants
antennas
surfactants
Surface-Active Agents
High Pressure Liquid Chromatography
Antennas

Keywords

  • Alpha-1-acid glycoprotein
  • Glycopeptide
  • Glycosylation
  • Mass spectrometry
  • RapiGest SF

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

Cite this

Glycosylation site analysis of human alpha-1-acid glycoprotein (AGP) by capillary liquid chromatography - Electrospray mass spectrometry. / Imre, T.; Schlosser, G.; Pocsfalvi, Gabriella; Siciliano, Rosa; Molnár-Szöllosi, Éva; Kremmer, T.; Malorni, Antonio; Vékey, K.

In: Journal of Mass Spectrometry, Vol. 40, No. 11, 11.2005, p. 1472-1483.

Research output: Contribution to journalArticle

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AU - Siciliano, Rosa

AU - Molnár-Szöllosi, Éva

AU - Kremmer, T.

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