Glycosaminoglycans: Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling

Derek J. Langeslay, Christopher J. Jones, S. Béni, Cynthia K. Larive

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Glycosaminoglycans (GAGs) are a class of biopolymers that include chondrotin sulfate, dermatan sulfate, keratan sulfate, hyaluronic acid, heparin, and heparan sulfate. The GAGs are linear polysaccharides that are microheterogeneous in composition and polydisperse in size. Because they have the most complex structures, this article is aimed at describing a step-by-step procedure for processing and analyzing heparin and heparan sulfate-derived oligosaccharides, although the basic protocols and procedures apply equally well to other members of the GAG family. The methods described in this manuscript include the preparation of oligosaccharides through enzymatic depolymerization, size fractionation by preparative scale size-exclusion chromatography (SEC), and disaccharide isomer analysis by reverse-phase ion-pair high-performance liquid chromatography (RPIP-HPLC) and capillary electrophoresis (CE).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages131-144
Number of pages14
Volume836
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume836
ISSN (Print)10643745

Fingerprint

Capillary Electrophoresis
Glycosaminoglycans
Oligosaccharides
Liquid Chromatography
Heparitin Sulfate
Heparin
Keratan Sulfate
Dermatan Sulfate
Biopolymers
Disaccharides
Hyaluronic Acid
Sulfates
Gel Chromatography
Polysaccharides
High Pressure Liquid Chromatography
Ions

Keywords

  • Anion-exchange
  • Compositional analysis
  • Depolymerization
  • Disaccharide
  • Glycosaminoglycan
  • Heparan sulfate
  • Heparin
  • Heparinase
  • Isomer
  • Oligosaccharide
  • RPIP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Langeslay, D. J., Jones, C. J., Béni, S., & Larive, C. K. (2012). Glycosaminoglycans: Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling. In Methods in Molecular Biology (Vol. 836, pp. 131-144). (Methods in Molecular Biology; Vol. 836). https://doi.org/10.1007/978-1-61779-498-8_9

Glycosaminoglycans : Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling. / Langeslay, Derek J.; Jones, Christopher J.; Béni, S.; Larive, Cynthia K.

Methods in Molecular Biology. Vol. 836 2012. p. 131-144 (Methods in Molecular Biology; Vol. 836).

Research output: Chapter in Book/Report/Conference proceedingChapter

Langeslay, DJ, Jones, CJ, Béni, S & Larive, CK 2012, Glycosaminoglycans: Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling. in Methods in Molecular Biology. vol. 836, Methods in Molecular Biology, vol. 836, pp. 131-144. https://doi.org/10.1007/978-1-61779-498-8_9
Langeslay DJ, Jones CJ, Béni S, Larive CK. Glycosaminoglycans: Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling. In Methods in Molecular Biology. Vol. 836. 2012. p. 131-144. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-498-8_9
Langeslay, Derek J. ; Jones, Christopher J. ; Béni, S. ; Larive, Cynthia K. / Glycosaminoglycans : Oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling. Methods in Molecular Biology. Vol. 836 2012. pp. 131-144 (Methods in Molecular Biology).
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