Glycoform heterogeneity of human serum α1-acid glycoprotein determined by CZE in malignant diseases

Krisztina Németh, T. Kremmer, László Kocsis, J. Visy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel capillary zone electrophoresis method was developed to investigate the glycoform heterogeneity of human serum α1-acid glycoprotein (AGP). The simultaneous application of a dimethyl polysiloxane coated capillary and oligoamine additives, particularly spermidine resulted in a more detailed separation of AGP glycoforms than reported previously. The relative distribution of AGP glycoforms in CZE was determined by baseline integration of peak areas and verified by peak-fitting analysis. Providing high purity of AGP samples suitable for CZE a schedule of isolation and purification steps including sample preparation and an improved technique of ion exchange chromatography was applied. Based on data obtained by CZE and on the serum AGP levels measured the serum concentrations of AGP glycoforms were calculated in cancer patients with Hodgkin and non-Hodgkin lymphoma, ovary carcinoma and melanoma compared to healthy donors. Results presented here demonstrated a significant increase in the serum concentration of the more acidic AGP fractions also indicating the overproduction of these glycoforms in cancer. In conclusion, our observations may raise the clinical diagnostic relevance of changes in the molecular heterogeneity of AGP detected by CZE in the various forms of malignant diseases.

Original languageEnglish
Pages (from-to)1307-1313
Number of pages7
JournalChromatographia
Volume69
Issue number11-12
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Glycoproteins
Acids
Serum
Spermidine
Ion Exchange Chromatography
Capillary Electrophoresis
Chromatography
Electrophoresis
Hodgkin Disease
Non-Hodgkin's Lymphoma
Purification
Ovary
Melanoma
Neoplasms
Ion exchange
Appointments and Schedules
Tissue Donors
Carcinoma

Keywords

  • Capillary zone electrophoresis
  • Glycoform heterogeneity in cancer
  • Human serum α1-acid glycoprotein
  • Ion exchange chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Glycoform heterogeneity of human serum α1-acid glycoprotein determined by CZE in malignant diseases. / Németh, Krisztina; Kremmer, T.; Kocsis, László; Visy, J.

In: Chromatographia, Vol. 69, No. 11-12, 06.2009, p. 1307-1313.

Research output: Contribution to journalArticle

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