Abstract
Horseradish essential oil (HREO; a natural mixture of different isothiocyanates) had strong fungicide effect against Candida albicans both in volatile and liquid phase. In liquid phase this antifungal effect was more significant than those of its main components allyl, and 2-phenylethyl isothiocyanate. HREO, at sublethal concentration, induced oxidative stress which was characterized with elevated superoxide content and up-regulated specific glutathione reductase, glutathione peroxidase, catalase and superoxide dismutase activities. Induction of specific glutathione S-transferase activities as marker of glutathione (GSH) dependent detoxification was also observed. At higher concentration, HREO depleted the GSH pool, increased heavily the superoxide production and killed the cells rapidly. HREO and the GSH pool depleting agent, 1-chlore-2,4-dinitrobenzene showed strong synergism when they were applied together to kill C. albicans cells. Based on all these, we assume that GSH metabolism protects fungi against isothiocyanates.
| Original language | English |
|---|---|
| Pages (from-to) | 1071-1079 |
| Number of pages | 9 |
| Journal | Journal of Basic Microbiology |
| Volume | 56 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2016 |
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Keywords
- Antifungal effects
- Candida albicans
- Glutathione metabolism
- Horseradish essential oil
- Isothiocyanates
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Glutathione protects Candida albicans against horseradish volatile oil. / Bertóti, Regina; Vasas, G.; Gonda, Sándor; Nguyen, Nhat Minh; Szőke, E.; Jakab, Ágnes; Pócsi, I.; Emri, T.
In: Journal of Basic Microbiology, Vol. 56, No. 10, 01.10.2016, p. 1071-1079.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glutathione protects Candida albicans against horseradish volatile oil
AU - Bertóti, Regina
AU - Vasas, G.
AU - Gonda, Sándor
AU - Nguyen, Nhat Minh
AU - Szőke, E.
AU - Jakab, Ágnes
AU - Pócsi, I.
AU - Emri, T.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Horseradish essential oil (HREO; a natural mixture of different isothiocyanates) had strong fungicide effect against Candida albicans both in volatile and liquid phase. In liquid phase this antifungal effect was more significant than those of its main components allyl, and 2-phenylethyl isothiocyanate. HREO, at sublethal concentration, induced oxidative stress which was characterized with elevated superoxide content and up-regulated specific glutathione reductase, glutathione peroxidase, catalase and superoxide dismutase activities. Induction of specific glutathione S-transferase activities as marker of glutathione (GSH) dependent detoxification was also observed. At higher concentration, HREO depleted the GSH pool, increased heavily the superoxide production and killed the cells rapidly. HREO and the GSH pool depleting agent, 1-chlore-2,4-dinitrobenzene showed strong synergism when they were applied together to kill C. albicans cells. Based on all these, we assume that GSH metabolism protects fungi against isothiocyanates.
AB - Horseradish essential oil (HREO; a natural mixture of different isothiocyanates) had strong fungicide effect against Candida albicans both in volatile and liquid phase. In liquid phase this antifungal effect was more significant than those of its main components allyl, and 2-phenylethyl isothiocyanate. HREO, at sublethal concentration, induced oxidative stress which was characterized with elevated superoxide content and up-regulated specific glutathione reductase, glutathione peroxidase, catalase and superoxide dismutase activities. Induction of specific glutathione S-transferase activities as marker of glutathione (GSH) dependent detoxification was also observed. At higher concentration, HREO depleted the GSH pool, increased heavily the superoxide production and killed the cells rapidly. HREO and the GSH pool depleting agent, 1-chlore-2,4-dinitrobenzene showed strong synergism when they were applied together to kill C. albicans cells. Based on all these, we assume that GSH metabolism protects fungi against isothiocyanates.
KW - Antifungal effects
KW - Candida albicans
KW - Glutathione metabolism
KW - Horseradish essential oil
KW - Isothiocyanates
UR - http://www.scopus.com/inward/record.url?scp=85027956550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027956550&partnerID=8YFLogxK
U2 - 10.1002/jobm.201600082
DO - 10.1002/jobm.201600082
M3 - Article
C2 - 27272511
AN - SCOPUS:85027956550
VL - 56
SP - 1071
EP - 1079
JO - Journal of Basic Microbiology
JF - Journal of Basic Microbiology
SN - 0233-111X
IS - 10
ER -