Glutathione protects Candida albicans against horseradish volatile oil

Regina Bertóti, G. Vasas, Sándor Gonda, Nhat Minh Nguyen, E. Szőke, Ágnes Jakab, I. Pócsi, T. Emri

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Horseradish essential oil (HREO; a natural mixture of different isothiocyanates) had strong fungicide effect against Candida albicans both in volatile and liquid phase. In liquid phase this antifungal effect was more significant than those of its main components allyl, and 2-phenylethyl isothiocyanate. HREO, at sublethal concentration, induced oxidative stress which was characterized with elevated superoxide content and up-regulated specific glutathione reductase, glutathione peroxidase, catalase and superoxide dismutase activities. Induction of specific glutathione S-transferase activities as marker of glutathione (GSH) dependent detoxification was also observed. At higher concentration, HREO depleted the GSH pool, increased heavily the superoxide production and killed the cells rapidly. HREO and the GSH pool depleting agent, 1-chlore-2,4-dinitrobenzene showed strong synergism when they were applied together to kill C. albicans cells. Based on all these, we assume that GSH metabolism protects fungi against isothiocyanates.

Original languageEnglish
Pages (from-to)1071-1079
Number of pages9
JournalJournal of Basic Microbiology
Volume56
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Armoracia
Isothiocyanates
Volatile Oils
Candida albicans
Superoxides
Glutathione
Glutathione Reductase
Glutathione Peroxidase
Glutathione Transferase
Catalase
Superoxide Dismutase
Oxidative Stress
Fungi

Keywords

  • Antifungal effects
  • Candida albicans
  • Glutathione metabolism
  • Horseradish essential oil
  • Isothiocyanates

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Glutathione protects Candida albicans against horseradish volatile oil. / Bertóti, Regina; Vasas, G.; Gonda, Sándor; Nguyen, Nhat Minh; Szőke, E.; Jakab, Ágnes; Pócsi, I.; Emri, T.

In: Journal of Basic Microbiology, Vol. 56, No. 10, 01.10.2016, p. 1071-1079.

Research output: Contribution to journalArticle

Bertóti, Regina ; Vasas, G. ; Gonda, Sándor ; Nguyen, Nhat Minh ; Szőke, E. ; Jakab, Ágnes ; Pócsi, I. ; Emri, T. / Glutathione protects Candida albicans against horseradish volatile oil. In: Journal of Basic Microbiology. 2016 ; Vol. 56, No. 10. pp. 1071-1079.
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