Glutathione in human melanoma cells. Effects of cysteine, cysteine esters and glutathione isopropyl ester

E. Karg, Anders Tunek, Harald Brötell, Anders Hallberg, Evald Rosengren, Hans Rorsman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Thiols are of great importance for the regulation of many cellular functions including metabolism, transport and cell protection. In this study the usefulness of l-cysteine methyl and octyl esters, of N,S-diacetyl-l-cysteine methyl ester and glutathione isopropyl ester as cellular cysteine and GSH delivery systems was investigated in the human IGR 1 melanoma cell line. The l-cysteine methyl and octyl esters proved to be highly toxic to the cells. Treatment of the cultures with 1 mM N,S-diacetyl-l-cysteine methyl ester or 3 mM glutathione isopropyl ester for 24 h resulted in marked elevation of the cellular glutathione level without apparent or with slight cell loss, respectively. Thus the administration of the latter two compounds seems to be suitable for inducing GSH elevation in the cultured melanoma cells.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalJournal of Dermatological Science
Volume1
Issue number1
DOIs
Publication statusPublished - 1990

Fingerprint

Glutathione
Cysteine
Melanoma
Esters
Diacetyl
Cytoprotection
Poisons
Sulfhydryl Compounds
Metabolism
Cultured Cells
Cells
Cell Line
mecysteine
glutathione monoisopropyl ester
cysteine octyl ester

Keywords

  • l-cysteine methyl ester
  • l-cysteine octyl ester
  • Thiols

ASJC Scopus subject areas

  • Dermatology

Cite this

Glutathione in human melanoma cells. Effects of cysteine, cysteine esters and glutathione isopropyl ester. / Karg, E.; Tunek, Anders; Brötell, Harald; Hallberg, Anders; Rosengren, Evald; Rorsman, Hans.

In: Journal of Dermatological Science, Vol. 1, No. 1, 1990, p. 39-45.

Research output: Contribution to journalArticle

Karg, E. ; Tunek, Anders ; Brötell, Harald ; Hallberg, Anders ; Rosengren, Evald ; Rorsman, Hans. / Glutathione in human melanoma cells. Effects of cysteine, cysteine esters and glutathione isopropyl ester. In: Journal of Dermatological Science. 1990 ; Vol. 1, No. 1. pp. 39-45.
@article{a7f2787cc0f1454caa4e9a355bc5a66d,
title = "Glutathione in human melanoma cells. Effects of cysteine, cysteine esters and glutathione isopropyl ester",
abstract = "Thiols are of great importance for the regulation of many cellular functions including metabolism, transport and cell protection. In this study the usefulness of l-cysteine methyl and octyl esters, of N,S-diacetyl-l-cysteine methyl ester and glutathione isopropyl ester as cellular cysteine and GSH delivery systems was investigated in the human IGR 1 melanoma cell line. The l-cysteine methyl and octyl esters proved to be highly toxic to the cells. Treatment of the cultures with 1 mM N,S-diacetyl-l-cysteine methyl ester or 3 mM glutathione isopropyl ester for 24 h resulted in marked elevation of the cellular glutathione level without apparent or with slight cell loss, respectively. Thus the administration of the latter two compounds seems to be suitable for inducing GSH elevation in the cultured melanoma cells.",
keywords = "l-cysteine methyl ester, l-cysteine octyl ester, Thiols",
author = "E. Karg and Anders Tunek and Harald Br{\"o}tell and Anders Hallberg and Evald Rosengren and Hans Rorsman",
year = "1990",
doi = "10.1016/0923-1811(90)90008-2",
language = "English",
volume = "1",
pages = "39--45",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Glutathione in human melanoma cells. Effects of cysteine, cysteine esters and glutathione isopropyl ester

AU - Karg, E.

AU - Tunek, Anders

AU - Brötell, Harald

AU - Hallberg, Anders

AU - Rosengren, Evald

AU - Rorsman, Hans

PY - 1990

Y1 - 1990

N2 - Thiols are of great importance for the regulation of many cellular functions including metabolism, transport and cell protection. In this study the usefulness of l-cysteine methyl and octyl esters, of N,S-diacetyl-l-cysteine methyl ester and glutathione isopropyl ester as cellular cysteine and GSH delivery systems was investigated in the human IGR 1 melanoma cell line. The l-cysteine methyl and octyl esters proved to be highly toxic to the cells. Treatment of the cultures with 1 mM N,S-diacetyl-l-cysteine methyl ester or 3 mM glutathione isopropyl ester for 24 h resulted in marked elevation of the cellular glutathione level without apparent or with slight cell loss, respectively. Thus the administration of the latter two compounds seems to be suitable for inducing GSH elevation in the cultured melanoma cells.

AB - Thiols are of great importance for the regulation of many cellular functions including metabolism, transport and cell protection. In this study the usefulness of l-cysteine methyl and octyl esters, of N,S-diacetyl-l-cysteine methyl ester and glutathione isopropyl ester as cellular cysteine and GSH delivery systems was investigated in the human IGR 1 melanoma cell line. The l-cysteine methyl and octyl esters proved to be highly toxic to the cells. Treatment of the cultures with 1 mM N,S-diacetyl-l-cysteine methyl ester or 3 mM glutathione isopropyl ester for 24 h resulted in marked elevation of the cellular glutathione level without apparent or with slight cell loss, respectively. Thus the administration of the latter two compounds seems to be suitable for inducing GSH elevation in the cultured melanoma cells.

KW - l-cysteine methyl ester

KW - l-cysteine octyl ester

KW - Thiols

UR - http://www.scopus.com/inward/record.url?scp=0025305741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025305741&partnerID=8YFLogxK

U2 - 10.1016/0923-1811(90)90008-2

DO - 10.1016/0923-1811(90)90008-2

M3 - Article

C2 - 2078540

AN - SCOPUS:0025305741

VL - 1

SP - 39

EP - 45

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 1

ER -