Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes

L. Braun, M. Csala, Anssi Poussu, Tamás Garzó, J. Mandl, G. Bánhegyi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonolactone could not be further affected by glutathione depletion, Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption. Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalFEBS Letters
Volume388
Issue number2-3
DOIs
Publication statusPublished - Jun 17 1996

Fingerprint

Glycogenolysis
Glutathione
Hepatocytes
Glutathione Disulfide
Glycogen
Diamide
Uridine Diphosphate Glucose
Vitamin K 3
Dithiothreitol
Fructose
Metabolism

Keywords

  • Ascorbate
  • Glutathione
  • Glycogenolysis
  • Mouse hepatocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes. / Braun, L.; Csala, M.; Poussu, Anssi; Garzó, Tamás; Mandl, J.; Bánhegyi, G.

In: FEBS Letters, Vol. 388, No. 2-3, 17.06.1996, p. 173-176.

Research output: Contribution to journalArticle

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AU - Bánhegyi, G.

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