Glutamate neurons are intermixed with midbrain dopamine neurons in nonhuman primates and humans

David H. Root, Hui Ling Wang, Bing Liu, David J. Barker, László Mód, Péter Szocsics, Afonso C. Silva, Z. Maglóczky, Marisela Morales

Research output: Contribution to journalArticle

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Abstract

The rodent ventral tegmental area (VTA) and substantia nigra pars compacta (SNC) contain dopamine neurons intermixed with glutamate neurons (expressing vesicular glutamate transporter 2; VGluT2), which play roles in reward and aversion. However, identifying the neuronal compositions of the VTA and SNC in higher mammals has remained challenging. Here, we revealed VGluT2 neurons within the VTA and SNC of nonhuman primates and humans by simultaneous detection of VGluT2 mRNA and tyrosine hydroxylase (TH; for identification of dopamine neurons). We found that several VTA subdivisions share similar cellular compositions in nonhuman primates and humans; their rostral linear nuclei have a high prevalence of VGluT2 neurons lacking TH; their paranigral and parabrachial pigmented nuclei have mostly TH neurons, and their parabrachial pigmented nuclei have dual VGluT2-TH neurons. Within nonhuman primates and humans SNC, the vast majority of neurons are TH neurons but VGluT2 neurons were detected in the pars lateralis subdivision. The demonstration that midbrain dopamine neurons are intermixed with glutamate or glutamate-dopamine neurons from rodents to humans offers new opportunities for translational studies towards analyzing the roles that each of these neurons play in human behavior and in midbrain-associated illnesses such as addiction, depression, schizophrenia, and Parkinson's disease.

Original languageEnglish
Number of pages1
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Aug 1 2016

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Dopaminergic Neurons
Mesencephalon
Primates
Glutamic Acid
Neurons
Ventral Tegmental Area
Rodentia
Vesicular Glutamate Transport Protein 2
Tyrosine 3-Monooxygenase
Reward
Parkinson Disease
Mammals
Schizophrenia
Depression
Messenger RNA
Pars Compacta

ASJC Scopus subject areas

  • General

Cite this

Root, D. H., Wang, H. L., Liu, B., Barker, D. J., Mód, L., Szocsics, P., ... Morales, M. (2016). Glutamate neurons are intermixed with midbrain dopamine neurons in nonhuman primates and humans. Scientific Reports, 6. https://doi.org/10.1038/srep30615

Glutamate neurons are intermixed with midbrain dopamine neurons in nonhuman primates and humans. / Root, David H.; Wang, Hui Ling; Liu, Bing; Barker, David J.; Mód, László; Szocsics, Péter; Silva, Afonso C.; Maglóczky, Z.; Morales, Marisela.

In: Scientific Reports, Vol. 6, 01.08.2016.

Research output: Contribution to journalArticle

Root, David H. ; Wang, Hui Ling ; Liu, Bing ; Barker, David J. ; Mód, László ; Szocsics, Péter ; Silva, Afonso C. ; Maglóczky, Z. ; Morales, Marisela. / Glutamate neurons are intermixed with midbrain dopamine neurons in nonhuman primates and humans. In: Scientific Reports. 2016 ; Vol. 6.
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