Glucose metabolism, hyperosmotic stress, and reprogramming of somatic cells

Rosalinda Madonna, A. Görbe, P. Ferdinándy, Raffaele De Caterina

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The availability of glucose and oxygen are important regulatory elements that help directing stem cell fate. In the undifferentiated state, stem cells, and their artificially reprogrammed equivalent-induced pluripotent stem cells (iPS) are characterized by limited oxidative capacity and active anaerobic glycolysis. Recent studies have shown that pluripotency - a characteristic of staminality - is associated with a poorly developed mitochondrial patrimony, while differentiation is accompanied by an activation of mitochondrial biogenesis. Besides being an important energy source in hypoxia, high glucose level results in hyperosmotic stress. The identification of specific metabolic pathways and biophysical factors that regulate stem cell fate, including high glucose in the extracellular medium, may therefore facilitate reprogramming efficiency and control the differentiation and fate of iPS cells, which are increasingly being explored as therapeutic tools. In this article, we review recent knowledge of the role of glucose metabolism and high glucose level as major anaerobic energy source, and a determinant of osmolarity as possible tools for reprogramming therapies in clinical applications. As in the diabetic setting hyperglycemia negatively affect the stem/progenitor cell fate and likely somatic reprogramming, we also discuss the in vivo potential transferability of the available in vitro findings.

Original languageEnglish
Pages (from-to)169-178
Number of pages10
JournalMolecular Biotechnology
Volume55
Issue number2
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Stem cells
Metabolism
Glucose
Stem Cells
Induced Pluripotent Stem Cells
Stem Cell Factor
Organelle Biogenesis
Glycolysis
Metabolic Networks and Pathways
Hyperglycemia
Osmolar Concentration
Chemical elements
Cellular Reprogramming
Oxygen
Chemical activation
Availability
Therapeutics

Keywords

  • Biophysical factors
  • Diabetes
  • Glucose
  • Hyperosmolarity
  • Induced pluripotent stem cells
  • Oxygen

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Glucose metabolism, hyperosmotic stress, and reprogramming of somatic cells. / Madonna, Rosalinda; Görbe, A.; Ferdinándy, P.; De Caterina, Raffaele.

In: Molecular Biotechnology, Vol. 55, No. 2, 10.2013, p. 169-178.

Research output: Contribution to journalArticle

Madonna, Rosalinda ; Görbe, A. ; Ferdinándy, P. ; De Caterina, Raffaele. / Glucose metabolism, hyperosmotic stress, and reprogramming of somatic cells. In: Molecular Biotechnology. 2013 ; Vol. 55, No. 2. pp. 169-178.
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