Glucose-mediated repression of autolysis and conidiogenesis in Emericella nidulans

T. Emri, Z. Molnár, Tünde Veres, T. Pusztahelyi, Gábor Dudás, I. Pócsi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Glucose-mediated repression of autolysis and sporulation was studied in submerged Emericella nidulans (anam. Aspergillus nidulans) cultures. Null mutation of the creA gene, which encodes the major carbon catabolite repressor CreA in E. nidulans, resulted in a hyperautolytic phenotype characterized by increased extracellular hydrolase production and dry cell mass declination. Interestingly, glucose, as well as the glucose antimetabolite 2-deoxy-d-glucose, repressed autolysis and sporulation in both the control and the creA null mutant strains suggesting that these processes were also subjected to CreA-independent carbon regulation. For example, the glucose-mediated, but CreA-independent, repression of the sporulation transcription factor BrlA was likely to contribute to the negative regulation of conidiogenesis by glucose. Although CreA played a prominent role in the regulation of autolysis via the repression of genes encoding important autolytic hydrolases like ChiB chitinase and PrtA protease the age-related production of the chitinase activity was also negatively affected by the down-regulation of brlA expression. However, neither CreA-dependent nor CreA-independent elements of carbon regulation affected the initiation and regulation of cell death in E. nidulans under carbon starvation.

Original languageEnglish
Pages (from-to)1172-1178
Number of pages7
JournalMycological Research
Volume110
Issue number10
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Emericella
Autolysis
autolysis
glucose
Glucose
sporulation
Carbon
Chitinases
carbon
Hydrolases
chitinase
hydrolases
Antimetabolites
Aspergillus nidulans
gene
Starvation
starvation
Genes
conidiation
repression

Keywords

  • Aspergillus nidulans
  • Chitinase
  • CreA
  • Proteinase
  • Sporulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Glucose-mediated repression of autolysis and conidiogenesis in Emericella nidulans. / Emri, T.; Molnár, Z.; Veres, Tünde; Pusztahelyi, T.; Dudás, Gábor; Pócsi, I.

In: Mycological Research, Vol. 110, No. 10, 10.2006, p. 1172-1178.

Research output: Contribution to journalArticle

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