Glucose-derived spiro-isoxazolines are anti-hyperglycemic agents against type 2 diabetes through glycogen phosphorylase inhibition

David Goyard, Bálint Kónya, Aikaterini S. Chajistamatiou, Evangelia D. Chrysina, Jérémy Leroy, Sophie Balzarin, Michel Tournier, Didier Tousch, Pierre Petit, Cédric Duret, Patrick Maurel, L. Somsák, T. Docsa, P. Gergely, Jean Pierre Praly, Jacqueline Azay-Milhau, Sébastien Vidal

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Glycogen phosphorylase (GP) is a target for the treatment of hyperglycaemia in the context of type 2 diabetes. This enzyme is responsible for the depolymerization of glycogen into glucose thereby affecting the levels of glucose in the blood stream. Twelve new d-glucopyranosylidene-spiro-isoxazolines have been prepared from O-peracylated exo-D-glucals by regio- and stereoselective 1,3-dipolar cycloaddition of nitrile oxides generated in situ by treatment of the corresponding oximes with bleach. This mild and direct procedure appeared to be applicable to a broad range of substrates. The corresponding O-unprotected spiro-isoxazolines were evaluated as glycogen phosphorylase (GP) inhibitors and exhibited IC50 values ranging from 1 to 800 μM. Selected inhibitors were further evaluated in vitro using rat and human hepatocytes and exhibited significant inhibitory properties in the primary cell culture. Interestingly, when tested with human hepatocytes, the tetra-O-acetylated spiro-isoxazoline bearing a 2-naphthyl residue showed a much lower IC50 value (2.5 μM), compared to that of the O-unprotected analog (19.95 μM). The most promising compounds were investigated in Zucker fa/fa rat model in acute and sub-chronic assays and decreased hepatic glucose production, which is known to be elevated in type 2 diabetes. This indicates that glucose-based spiro-isoxazolines can be considered as anti-hyperglycemic agents in the context of type 2 diabetes.

Original languageEnglish
Pages (from-to)444-454
Number of pages11
JournalEuropean Journal of Medicinal Chemistry
Volume108
DOIs
Publication statusPublished - Jan 27 2016

Keywords

  • Carbohydrates
  • Glycogen phosphorylase inhibitors
  • Hepatic glucose production
  • Hepatocytes
  • Spiro-isoxazolines
  • Type 2 diabetes
  • Zucker rats

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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    Goyard, D., Kónya, B., Chajistamatiou, A. S., Chrysina, E. D., Leroy, J., Balzarin, S., Tournier, M., Tousch, D., Petit, P., Duret, C., Maurel, P., Somsák, L., Docsa, T., Gergely, P., Praly, J. P., Azay-Milhau, J., & Vidal, S. (2016). Glucose-derived spiro-isoxazolines are anti-hyperglycemic agents against type 2 diabetes through glycogen phosphorylase inhibition. European Journal of Medicinal Chemistry, 108, 444-454. https://doi.org/10.1016/j.ejmech.2015.12.004