Glucose-6-phosphatase in the insulin secreting cell line INS-1

Rosella Fulceri, Tamás Kardon, Gábor Bánhegyi, William F. Pralong, Alessandra Gamberucci, Paola Marcolongo, Angelo Benedetti

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Abstract

The glucose-6-phosphatase system of the glucose sensitive insulin secreting rat insulinoma cells (INS-1) was investigated. INS-1 cells contain easily detectable levels of glucose-6-phosphatase enzyme protein (assessed by Western blotting) and have a very significant enzymatic activity. The features of the enzyme (Km and Vmax values, sensitivity to acidic pH, partial latency, and double immunoreactive band) are similar to those of the hepatic form. On the other hand, hardly detectable levels of glucose-6-phosphatase activity and protein were present in the parent glucose insensitive RINm5F cell line. The mRNA of the glucose-6-phosphate transporter was also more abundant in the INS-1 cells. The results support the view that the glucose-6-phosphatase system of the β-cell is associated with the regulation of insulin secretion. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalBiochemical and biophysical research communications
Volume275
Issue number1
DOIs
Publication statusPublished - Aug 18 2000

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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