Gluconeogenesis from ascorbic acid: Ascorbate recycling in isolated murine hepatocytes

László Braun, Ferenc Puskás, Miklós Csala, Erika Gyorffy, Tamás Garzó, József Mandl, Gábor Bánhegyi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ascorbic acid synthesis and breakdown were investigated in isolated hepatocytes prepared from fasted mice. Stimulation of gluconeogenesis by alanine or xylitol led to ascorbate synthesis. On the other hand, ascorbate or dehydroascorbate addition resulted in concentration-dependent glucose production and elevation of the pentose phosphate pathway intermediate xylulose 5-phosphate. Stimulation of ascorbate oxidation and/or the inhibition of dehydroascorbate reduction increased glucose formation. Inhibition of the pentose phosphate pathway decreased glucose production from dehydroascorbate with increased accumulation of xylulose 5-phosphate. These results suggest that ascorbate can be recycled by a novel way involving intermediates of the pentose phosphate pathway, gluconeogenesis and hexuronic acid pathway.

Original languageEnglish
Pages (from-to)183-186
Number of pages4
JournalFEBS letters
Volume390
Issue number2
DOIs
Publication statusPublished - Jul 22 1996

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Keywords

  • Ascorbic acid
  • Dehydroascorbic acid
  • Gluconeogenesis
  • Glutathione
  • Isolated hepatocyte
  • Mouse
  • Pentose phosphate pathway

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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