Glucocorticoid receptor protein expression in human hippocampus; stability with age

Qian Wang, Joop Van Heerikhuize, Eleonora Aronica, Mitsuhiro Kawata, L. Séress, Marian Joels, Dick F. Swaab, Paul J. Lucassen

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The glucocorticoid receptor (GR) exerts numerous functions in the body and brain. In the brain, it has been implicated, amongst others, in feedback regulation of the hypothalamic-pituitary-adrenal axis, with potential deficits during aging and in depression. GRs are abundantly expressed in the hippocampus of rodent, except for the Ammon's horn (CA) 3 subregion. In rhesus monkey however, GR protein was largely absent from all hippocampal subregions, which prompted us to investigate its distribution in human hippocampus. After validation of antibody specificity, we investigated GRα protein distribution in the postmortem hippocampus of 26 human control subjects (1-98 years of age) and quantified changes with age and sex. In contrast to monkey, abundant GR-immunoreactivity was present in nuclei of almost all neurons of the hippocampal CA subfields and dentate gyrus (DG), although neurons of the CA3 subregion displayed lower levels of immunoreactivity. Colocalization with glial fibrillary acidic protein confirmed that GR was additionally expressed in approximately 50% of the astrocytes in the CA regions, with lower levels of colocalization (approximately 20%) in the DG. With increased age, GR expression remained stable in the CA regions in both sexes, whereas a significant negative correlation was found with age only in the DG of females. Thus, in contrast to the very low levels previously reported in monkey, GR protein is prominently expressed in human hippocampus, indicating that this region can form an important target for corticosteroid effects in human.

Original languageEnglish
Pages (from-to)1662-1673
Number of pages12
JournalNeurobiology of Aging
Volume34
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Glucocorticoid Receptors
Hippocampus
Dentate Gyrus
Proteins
Haplorhini
Neurons
Antibody Specificity
Glial Fibrillary Acidic Protein
Brain
Macaca mulatta
Astrocytes
Rodentia
Adrenal Cortex Hormones

Keywords

  • Glucocorticoid receptor
  • Hippocampus
  • Human brain
  • Hypothalamus
  • Immunocytochemistry

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Ageing
  • Developmental Biology
  • Geriatrics and Gerontology

Cite this

Wang, Q., Van Heerikhuize, J., Aronica, E., Kawata, M., Séress, L., Joels, M., ... Lucassen, P. J. (2013). Glucocorticoid receptor protein expression in human hippocampus; stability with age. Neurobiology of Aging, 34(6), 1662-1673. https://doi.org/10.1016/j.neurobiolaging.2012.11.019

Glucocorticoid receptor protein expression in human hippocampus; stability with age. / Wang, Qian; Van Heerikhuize, Joop; Aronica, Eleonora; Kawata, Mitsuhiro; Séress, L.; Joels, Marian; Swaab, Dick F.; Lucassen, Paul J.

In: Neurobiology of Aging, Vol. 34, No. 6, 06.2013, p. 1662-1673.

Research output: Contribution to journalArticle

Wang, Q, Van Heerikhuize, J, Aronica, E, Kawata, M, Séress, L, Joels, M, Swaab, DF & Lucassen, PJ 2013, 'Glucocorticoid receptor protein expression in human hippocampus; stability with age', Neurobiology of Aging, vol. 34, no. 6, pp. 1662-1673. https://doi.org/10.1016/j.neurobiolaging.2012.11.019
Wang, Qian ; Van Heerikhuize, Joop ; Aronica, Eleonora ; Kawata, Mitsuhiro ; Séress, L. ; Joels, Marian ; Swaab, Dick F. ; Lucassen, Paul J. / Glucocorticoid receptor protein expression in human hippocampus; stability with age. In: Neurobiology of Aging. 2013 ; Vol. 34, No. 6. pp. 1662-1673.
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