Glucocorticoid receptor in magnocellular neurosecretory cells

J. Kiss, J. A M Van Eekelen, J. M H M Reul, H. M. Westphal, E. R. De Kloet

Research output: Contribution to journalArticle

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Abstract

Quantitative in vitro autoradiography, cytosol receptor assay in punched brain tissue, and immunocytochemistry have revealed that the glucocorticoid receptor is present in the rat supraoptic nucleus (SON). Based on its binding characteristics the receptor appears to be the type II glucocorticoid receptor. With the use of a monoclonal antibody against purified liver glucocorticoid receptor, immunostaining was found in magnocellular neurosecretory neurons in the SON, but not in magnocellular neurons in the paraventricular nucleus. Immunoreactive cells seem to be concentrated in ventral parts of the SON where vasopressin cells were previously shown to be located. One to 2 weeks after bilateral adrenalectomy, there was a substantial decrease in glucocorticoid receptor immunostaining in magnocellular as well as other types of neurons in various brain regions. Administration of synthetic glucocorticoids (RU 28362 or dexamethasone) induced a robust increase in the intensity of immunostaining in cell nuclei of neurosecretory cells. The presence of glucocorticoid receptors in the SON suggests that glucocorticoids may affect vasopressin synthesis or/and secretion through a direct action of magnocellular neurons.

Original languageEnglish
Pages (from-to)444-449
Number of pages6
JournalEndocrinology
Volume122
Issue number2
Publication statusPublished - 1988

Fingerprint

Glucocorticoid Receptors
Supraoptic Nucleus
Neurons
Vasopressins
Glucocorticoids
Paraventricular Hypothalamic Nucleus
Adrenalectomy
Brain
Autoradiography
Cell Nucleus
Cytosol
Dexamethasone
Immunohistochemistry
Monoclonal Antibodies
Liver

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Kiss, J., Van Eekelen, J. A. M., Reul, J. M. H. M., Westphal, H. M., & De Kloet, E. R. (1988). Glucocorticoid receptor in magnocellular neurosecretory cells. Endocrinology, 122(2), 444-449.

Glucocorticoid receptor in magnocellular neurosecretory cells. / Kiss, J.; Van Eekelen, J. A M; Reul, J. M H M; Westphal, H. M.; De Kloet, E. R.

In: Endocrinology, Vol. 122, No. 2, 1988, p. 444-449.

Research output: Contribution to journalArticle

Kiss, J, Van Eekelen, JAM, Reul, JMHM, Westphal, HM & De Kloet, ER 1988, 'Glucocorticoid receptor in magnocellular neurosecretory cells', Endocrinology, vol. 122, no. 2, pp. 444-449.
Kiss J, Van Eekelen JAM, Reul JMHM, Westphal HM, De Kloet ER. Glucocorticoid receptor in magnocellular neurosecretory cells. Endocrinology. 1988;122(2):444-449.
Kiss, J. ; Van Eekelen, J. A M ; Reul, J. M H M ; Westphal, H. M. ; De Kloet, E. R. / Glucocorticoid receptor in magnocellular neurosecretory cells. In: Endocrinology. 1988 ; Vol. 122, No. 2. pp. 444-449.
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