Glucocorticoid Homeostasis in the Dentate Gyrus Is Essential for Opiate Withdrawal-Associated Memories

Daniel García-Pérez, Szilamer Ferenczi, K. Kovács, M. Luisa Laorden, M. Victoria Milanés, Cristina Núñez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Drug-withdrawal-associated aversive memories might trigger relapse to drug-seeking behavior. However, changes in structural and synaptic plasticity, as well as epigenetic mechanisms, which may be critical for long-term aversive memory, have yet to be elucidated. We used male Wistar rats and performed conditioned-place aversion (CPA) paradigm to uncover the role of glucocorticoids (GCs) on plasticity-related processes that occur within the dentate gyrus (DG) during opiate-withdrawal conditioning (memory formation-consolidation) and after reactivation by re-exposure to the conditioned environment (memory retrieval). Rats subjected to conditioned morphine-withdrawal robustly expressed CPA, while adrenalectomy impaired naloxone-induced CPA. Importantly, while activity-regulated cytoskeletal-associated protein (Arc) expression was induced in sham- and ADX-dependent animals during the conditioning phase, Arc and early growth response 1 (Egr-1) induction was restricted to sham-dependent rats following memory retrieval. Moreover, we found a correlation between Arc induction and CPA score, and Arc was selectively expressed in the granular zone of the DG in dopaminoceptive, glutamatergic and GABAergic neurons. We further found that brain-derived neurotrophic factor was regulated in the opposite way during the test phase. Our results also suggest a role for epigenetic regulation on the expression of glucocorticoid receptors and Arc following memory retrieval. Our data provide the first evidence that GC homeostasis is important for the expression of long-term morphine-withdrawal memories. Moreover, our results support the idea that targeting Arc and Egr-1 in the DG may provide important insights into the role of these signaling cascades in withdrawal-context memory re-consolidation. Together, disrupting these processes in the DG might lead to effective treatments in drug addiction thereby rapidly and persistently reducing invasive memories and subsequent drug seeking.

Original languageEnglish
Pages (from-to)1-19
Number of pages19
JournalMolecular Neurobiology
DOIs
Publication statusAccepted/In press - Oct 11 2016

Fingerprint

Opiate Alkaloids
Dentate Gyrus
Glucocorticoids
Homeostasis
Epigenomics
Morphine
Drug-Seeking Behavior
GABAergic Neurons
Neuronal Plasticity
Long-Term Memory
Adrenalectomy
Brain-Derived Neurotrophic Factor
Glucocorticoid Receptors
Naloxone
Growth
Pharmaceutical Preparations
Substance-Related Disorders
activity regulated cytoskeletal-associated protein
Wistar Rats
Recurrence

Keywords

  • Adrenalectomy
  • Conditioned-place aversion
  • Memory consolidation and retrieval
  • miRNAs
  • Morphine dependence
  • Transcription factors

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Glucocorticoid Homeostasis in the Dentate Gyrus Is Essential for Opiate Withdrawal-Associated Memories. / García-Pérez, Daniel; Ferenczi, Szilamer; Kovács, K.; Laorden, M. Luisa; Milanés, M. Victoria; Núñez, Cristina.

In: Molecular Neurobiology, 11.10.2016, p. 1-19.

Research output: Contribution to journalArticle

García-Pérez, Daniel ; Ferenczi, Szilamer ; Kovács, K. ; Laorden, M. Luisa ; Milanés, M. Victoria ; Núñez, Cristina. / Glucocorticoid Homeostasis in the Dentate Gyrus Is Essential for Opiate Withdrawal-Associated Memories. In: Molecular Neurobiology. 2016 ; pp. 1-19.
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