Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression

Nándor Lipták, Orsolya Ivett Hoffmann, Gabriella Skoda, Elen Gócza, Andrea Kerekes, Zsuzsanna Bősze, László Hiripi

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Abstract

Focal segmental glomerulosclerosis (FSGS) is a potential cause of nephrotic syndrome both in humans and pet mammals. Glomerulopathy was reported earlier in green fluorescent protein (GFP) transgenic (TG) mice, but glomerulosclerosis has not been examined in GFP TG rabbits so far. In the present study, the potential manifestation of FSGS was investigated in both Venus TG rabbits generated by Sleeping Beauty (SB) transposition and age-matched control New Zealand White (NZW) rabbits. Venus protein fluorescence was detected by confocal microscopy and quantified by microplate reader. Urinalysis, haematology, serum biochemistry and renal histology were performed to assess the signs of FSGS. Higher levels of Venus fluorescence were determined in renal cortex samples than in the myocardium by both methods. Urinalysis revealed proteinuria in Venus heterozygote TG bucks, while Venus homozygote TG bucks developed microscopic haematuria. Supporting the urinalysis data, the histological findings of FSGS (glomerulomegaly and sclerotic glomeruli) were observed in renal cortex sections of Venus TG rabbits. Taken together, Venus TG bucks were diagnosed with FSGS; thus, this type of glomerulopathy could be a common disease in TG animals overexpressing GFP.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalActa veterinaria Hungarica
Volume66
Issue number2
DOIs
Publication statusPublished - Jun 2018

Keywords

  • Focal segmental glomerulosclerosis
  • Transgenic rabbits
  • Urinalysis

ASJC Scopus subject areas

  • veterinary(all)

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  • Cite this

    Lipták, N., Hoffmann, O. I., Skoda, G., Gócza, E., Kerekes, A., Bősze, Z., & Hiripi, L. (2018). Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression. Acta veterinaria Hungarica, 66(2), 281-293. https://doi.org/10.1556/004.2018.026