Global organization of metabolic fluxes in the bacterium Escherichia coli

E. Almaas, B. Kovács, T. Vicsek, Z. N. Oltvai, A. L. Barabási

Research output: Contribution to journalArticle

510 Citations (Scopus)

Abstract

Cellular metabolism, the integrated interconversion of thousands of metabolic substrates through enzyme-catalysed biochemical reactions, is the most investigated complex intracellular web of molecular interactions. Although the topological organization of individual reactions into metabolic networks is well understood, the principles that govern their global functional use under different growth conditions raise many unanswered questions. By implementing a flux balance analysis of the metabolism of Escherichia coli strain MG1655, here we show that network use is highly uneven. Whereas most metabolic reactions have low fluxes, the overall activity of the metabolism is dominated by several reactions with very high fluxes. E. coli responds to changes in growth conditions by reorganizing the rates of selected fluxes predominantly within this high-flux backbone. This behaviour probably represents a universal feature of metabolic activity in all cells, with potential implications for metabolic engineering.

Original languageEnglish
Pages (from-to)839-843
Number of pages5
JournalNature
Volume427
Issue number6977
DOIs
Publication statusPublished - Feb 26 2004

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Escherichia coli
Bacteria
Metabolic Engineering
Growth
Metabolic Networks and Pathways
Enzymes

ASJC Scopus subject areas

  • General

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Almaas, E., Kovács, B., Vicsek, T., Oltvai, Z. N., & Barabási, A. L. (2004). Global organization of metabolic fluxes in the bacterium Escherichia coli. Nature, 427(6977), 839-843. https://doi.org/10.1038/nature02289

Global organization of metabolic fluxes in the bacterium Escherichia coli. / Almaas, E.; Kovács, B.; Vicsek, T.; Oltvai, Z. N.; Barabási, A. L.

In: Nature, Vol. 427, No. 6977, 26.02.2004, p. 839-843.

Research output: Contribution to journalArticle

Almaas, E, Kovács, B, Vicsek, T, Oltvai, ZN & Barabási, AL 2004, 'Global organization of metabolic fluxes in the bacterium Escherichia coli', Nature, vol. 427, no. 6977, pp. 839-843. https://doi.org/10.1038/nature02289
Almaas, E. ; Kovács, B. ; Vicsek, T. ; Oltvai, Z. N. ; Barabási, A. L. / Global organization of metabolic fluxes in the bacterium Escherichia coli. In: Nature. 2004 ; Vol. 427, No. 6977. pp. 839-843.
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