Glial responses to neonatal hypoxic-ischemic injury in the rat cerebral cortex

Stéphane V. Sizonenko, Emily J. Camm, Alexandre Dayer, J. Kiss

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Neurogenesis is nearly completed after birth, whereas gliogenic activities remain intense during the postnatal period in the developing rat cortex. These include involution of radial glia, proliferation of astrocytes and oligodendrocytes and myelin formation. Little is known about the effects of hypoxic-ischemic (HI) injury on these critical postnatal processes. Here we explored the glial reactions to mild HI injury of the neonatal rat cerebral cortex at P3. We show that the HI lesion results in disruption of the normal radial glia architecture, which was paralleled by an increase in GFAP immunopositive reactive astrocytes. The morphology of these latter cells and the fact that they were immunolabelled for both nestin and GFAP suggest an accelerated transformation of radial glia into astrocytes. In addition, BrdU/GFAP immunostaining revealed a significant increase of double-labelled cells indicating an acute proliferation of astrocytes after HI. This enhanced proliferative activity of astrocytes persisted for several weeks. We found an elevated number and increased mitotic activity of both NG2-positive oligodendrocyte progenitors and RIP-positive oligodendrocytes after injury. These findings imply that glial responses are central to cortical tissue remodelling following neonatal ischemia and represent a potential target for therapeutic approaches.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Neuroglia
Astrocytes
Cerebral Cortex
Oligodendroglia
Wounds and Injuries
Nestin
Neurogenesis
Bromodeoxyuridine
Myelin Sheath
Ischemia
Parturition
Therapeutics

Keywords

  • Astrocyte
  • Brain injury
  • Gliosis
  • Hypoxic-ischemic
  • Oligodendrocyte
  • Radial glia
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Glial responses to neonatal hypoxic-ischemic injury in the rat cerebral cortex. / Sizonenko, Stéphane V.; Camm, Emily J.; Dayer, Alexandre; Kiss, J.

In: International Journal of Developmental Neuroscience, Vol. 26, No. 1, 02.2008, p. 37-45.

Research output: Contribution to journalArticle

Sizonenko, Stéphane V. ; Camm, Emily J. ; Dayer, Alexandre ; Kiss, J. / Glial responses to neonatal hypoxic-ischemic injury in the rat cerebral cortex. In: International Journal of Developmental Neuroscience. 2008 ; Vol. 26, No. 1. pp. 37-45.
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