Glial architecture of the ghost shark (Callorhinchus milii, Holocephali, chondrichthyes) as revealed by different immunohistochemical markers

Csilla Ari, M. Kálmán

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This article presents the first study on the glial architecture of a representative species of Holocephali, Callorhinchus milii (ghost shark). Holocephali are a small subclass of Chondrichthyes, with only a few extant genera, and those are considered to have a brain organization more similar to squalomorph sharks than to galeomorph sharks, skates, and rays. Three different astroglial markers - glial fibrillary acidic protein, S-100 protein, and glutamine synthetase (GS) - were investigated by immunohistochemical methods, applying both diaminobenzidine (DAB) and fluorescent techniques. They revealed similar glial structures, although most of them were detected by immunohistochemical reaction against GS and visualized by DAB. The predominant elements were radial ependymoglia spanning the area between the ventricular and meningeal surfaces, as in squalomorph sharks. Other similar features were the light appearance of myelinated neural tracts devoid of immunoreactivity, and the glial architecture of the reticular formation of the brain stem, cerebellum, and tectum, the latter with recognizable layers. The immunoreactivity of the vascular walls was similar; however, it is believed that different cell types form the blood-brain barrier in chimeras and in elasmobranchs. Some glial structures, however, resembled those of skates, rays, and galeomorph sharks. In C. milii astrocyte-like elements were observed in the telencephalon, using GS and S-100, although typical astrocyte-rich regions were not found. In some areas, especially the telencephalon, not only endfeet but also cell bodies were observed to be attached to the meningeal surface, with processes extending into the brain substance.

Original languageEnglish
Pages (from-to)504-519
Number of pages16
JournalJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume310
Issue number6
DOIs
Publication statusPublished - Sep 15 2008

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Sharks
Chondrichthyes
neuroglia
shark
sharks
Neuroglia
Glutamate-Ammonia Ligase
brain
glutamate-ammonia ligase
Telencephalon
Rajidae
astrocytes
Astrocytes
Elasmobranchii
chimera
Reticular Formation
protein
blood-brain barrier
S100 Proteins
chimerism

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "Glial architecture of the ghost shark (Callorhinchus milii, Holocephali, chondrichthyes) as revealed by different immunohistochemical markers",
abstract = "This article presents the first study on the glial architecture of a representative species of Holocephali, Callorhinchus milii (ghost shark). Holocephali are a small subclass of Chondrichthyes, with only a few extant genera, and those are considered to have a brain organization more similar to squalomorph sharks than to galeomorph sharks, skates, and rays. Three different astroglial markers - glial fibrillary acidic protein, S-100 protein, and glutamine synthetase (GS) - were investigated by immunohistochemical methods, applying both diaminobenzidine (DAB) and fluorescent techniques. They revealed similar glial structures, although most of them were detected by immunohistochemical reaction against GS and visualized by DAB. The predominant elements were radial ependymoglia spanning the area between the ventricular and meningeal surfaces, as in squalomorph sharks. Other similar features were the light appearance of myelinated neural tracts devoid of immunoreactivity, and the glial architecture of the reticular formation of the brain stem, cerebellum, and tectum, the latter with recognizable layers. The immunoreactivity of the vascular walls was similar; however, it is believed that different cell types form the blood-brain barrier in chimeras and in elasmobranchs. Some glial structures, however, resembled those of skates, rays, and galeomorph sharks. In C. milii astrocyte-like elements were observed in the telencephalon, using GS and S-100, although typical astrocyte-rich regions were not found. In some areas, especially the telencephalon, not only endfeet but also cell bodies were observed to be attached to the meningeal surface, with processes extending into the brain substance.",
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