Gingival endothelial and inducible nitric oxide synthase levels during orthodontic treatment: A cross-sectional study

Michele D'Attillio, Franca Di Maio, Camillo D'Arcangela, Maria Rita Filippi, Mario Felaco, Zs. Lohinai, Felice Festa, Giuseppe Perinetti

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study uses a cross-sectional design to examine the endothelial and inducible nitric oxide synthase (eNOS and iNOS, respectively) levels of gingival tissue. Fifteen subjects, 10 female and 5 male individuals (aged 14.6-21.2 years; mean 17.4 ± 1.8 years), who needed extraction of the four first premolars for orthodontic reasons and who had indications for a gingivectomy were enrolled in the study. In each patient, two maxillary/mandibular premolars were extracted, and two months later an orthodontic appliance was placed in the same arch. A canine undergoing treatment for distal movement served as the test tooth (TT), whereas its contralateral canine was used as the control tooth (CT). The CT was included in the orthodontic appliance but was not subjected to the orthodontic force. Two weeks after the orthodontic appliance placement, clinical data consisting of the presence of supragingival plaque, bleeding on probing, and probing depth were collected from each experimental tooth. Immediately after, gingival tissue was collected from the distal aspect of each TT and CT for immunohistochemistry, messenger RNA reverse transcription by polymerase chain reaction, and Western blot analysis for both eNOS and iNOS. The results showed that no differences in clinical conditions occurred between the experimental teeth. On the contrary, both the eNOS and iNOS levels and the expression of the TTs were significantly greater than those of the CTs (all comparisons significant to P <.01). Our results indicate a role for gingival eNOS and iNOS during the early phases of orthodontic treatment in humans.

Original languageEnglish
Pages (from-to)851-858
Number of pages8
JournalAngle Orthodontist
Volume74
Issue number6
Publication statusPublished - Dec 2004

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Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type II
Orthodontics
Tooth
Cross-Sectional Studies
Orthodontic Appliances
Bicuspid
Therapeutics
Canidae
Gingivectomy
Reverse Transcription
Western Blotting
Immunohistochemistry
Hemorrhage
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Nitric oxide synthase
  • Orthodontic tooth movement
  • Tissue remodeling/inflammation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

D'Attillio, M., Di Maio, F., D'Arcangela, C., Filippi, M. R., Felaco, M., Lohinai, Z., ... Perinetti, G. (2004). Gingival endothelial and inducible nitric oxide synthase levels during orthodontic treatment: A cross-sectional study. Angle Orthodontist, 74(6), 851-858.

Gingival endothelial and inducible nitric oxide synthase levels during orthodontic treatment : A cross-sectional study. / D'Attillio, Michele; Di Maio, Franca; D'Arcangela, Camillo; Filippi, Maria Rita; Felaco, Mario; Lohinai, Zs.; Festa, Felice; Perinetti, Giuseppe.

In: Angle Orthodontist, Vol. 74, No. 6, 12.2004, p. 851-858.

Research output: Contribution to journalArticle

D'Attillio, M, Di Maio, F, D'Arcangela, C, Filippi, MR, Felaco, M, Lohinai, Z, Festa, F & Perinetti, G 2004, 'Gingival endothelial and inducible nitric oxide synthase levels during orthodontic treatment: A cross-sectional study', Angle Orthodontist, vol. 74, no. 6, pp. 851-858.
D'Attillio, Michele ; Di Maio, Franca ; D'Arcangela, Camillo ; Filippi, Maria Rita ; Felaco, Mario ; Lohinai, Zs. ; Festa, Felice ; Perinetti, Giuseppe. / Gingival endothelial and inducible nitric oxide synthase levels during orthodontic treatment : A cross-sectional study. In: Angle Orthodontist. 2004 ; Vol. 74, No. 6. pp. 851-858.
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