Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)

Carmen Gallas-Lindemann, Isaia Sotiriadou, J. Plutzer, Michael J. Noack, Mohammad Reza Mahmoudi, Panagiotis Karanis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n = 185) and Cryptosporidium spp. (n = 227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalActa Tropica
Volume158
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Giardia
Cryptosporidium
Waste Water
wastewater treatment
fluorescent antibody technique
Fluorescent Antibody Technique
polymerase chain reaction
Polymerase Chain Reaction
assays
Flocculation
sampling
Oocysts
Water
Therapeutics
Centrifugation
Germany
Sucrose
Cysts
loop-mediated isothermal amplification
DNA

Keywords

  • Cryptosporidium
  • Detection
  • Dissemination
  • Giardia
  • LAMP
  • Wastewater

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). / Gallas-Lindemann, Carmen; Sotiriadou, Isaia; Plutzer, J.; Noack, Michael J.; Mahmoudi, Mohammad Reza; Karanis, Panagiotis.

In: Acta Tropica, Vol. 158, 01.06.2016, p. 43-51.

Research output: Contribution to journalArticle

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