Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

M. Gálfi, M. Radács, Z. Molnár, I. Budai, G. Tóth, A. Pósa, K. Kupai, Z. Szalai, R. Szabó, H. A. Molnár, J. Gardi, F. László, C. Varga

Research output: Contribution to journalArticle

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Abstract

The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13–14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of vasopressin and oxytocin production were detected in the cell culture media following ghrelin administration, depending on the ghrelin doses. The oxytocin level proved to be more elevated than that of vasopressin. The increase of vasopressin and oxytocin secretion could be totally blocked by previous administration of the ghrelin receptor antagonist ([D-Lys3]-growth hormone-releasing peptide-6). Application of the ghrelin receptor antagonist after ghrelin administration proved ineffective. The results indicate that vasopressin and oxytocin release is influenced directly by the ghrelin system, and the effects of ghrelin on vasopressin and oxytocin secretion from the neurohypophyseal tissue in rats can occur at the level of the posterior pituitary. Our observations lend support to the view that neurohypophysis contains ghrelin receptors.

Original languageEnglish
Pages (from-to)525-530
Number of pages6
JournalJournal of Molecular Neuroscience
Volume60
Issue number4
DOIs
Publication statusPublished - Dec 1 2016

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Ghrelin
Oxytocin
Vasopressins
Cell Culture Techniques
Ghrelin Receptor
Posterior Pituitary Gland
Radioimmunoassay
Culture Media

Keywords

  • Ghrelin
  • Ghrelin receptor antagonist
  • Neurohypophyseal cell culture
  • Oxytocin
  • Vasopressin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures. / Gálfi, M.; Radács, M.; Molnár, Z.; Budai, I.; Tóth, G.; Pósa, A.; Kupai, K.; Szalai, Z.; Szabó, R.; Molnár, H. A.; Gardi, J.; László, F.; Varga, C.

In: Journal of Molecular Neuroscience, Vol. 60, No. 4, 01.12.2016, p. 525-530.

Research output: Contribution to journalArticle

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