Germ line specific expression of a protein phosphatase Y interacting protein (PPYR1) in Drosophila

Endre Kókai, Milán Szuperák, Luke Alphey, János Gausz, Géza Ádám, V. Dombrádi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

PPYR1, the product of the CG15031 gene, was identified as a protein phosphatase Y (PPY) interacting protein in Drosophila melanogaster using a yeast two-hybrid screen. PPYR1 displays a biphasic expression pattern: the maternal protein is abundant in the developing egg chambers and in the early embryos, while the zygotic protein appears later in development and is localized specifically in the testes of the males. The maternal and zygotic gene products differ from each other in their size having apparent molecular masses of 47 and 66 kDa, respectively. The maternal PPYR1 is localized in the cytoplasm of the follicular and nurse cells and is deposited as a ribonucleoprotein complex in the oocyte. In the early embryos, the PPYR1 is distributed evenly, and it gradually diminishes during embryonic development. Zygotic PPYR1 is expressed exclusively in the testes, predominantly in the cytoplasm of the spermatocytes. PPY is localized in the nuclei of the same cells. Our results suggest that PPYR1 has two distinct developmental isoforms: a maternal protein the expression of which is independent of PPY and a zygotic protein which is co-expressed with PPY.

Original languageEnglish
Pages (from-to)724-729
Number of pages6
JournalGene Expression Patterns
Volume6
Issue number7
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Phosphoprotein Phosphatases
Germ Cells
Mothers
Testis
Cytoplasm
Proteins
Embryonic Structures
Drosophila Proteins
Ribonucleoproteins
Spermatocytes
Cell Nucleus
Genes
Oocytes
Embryonic Development
Ovum
Protein Isoforms
Yeasts
Nurses
Drosophila inturned protein
Drosophila CG15031 protein

Keywords

  • CG15031
  • Drosophila melanogaster
  • Oocyte
  • Protein phosphatase Y interacting protein
  • Spermatocyte
  • Subcellular localization

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Germ line specific expression of a protein phosphatase Y interacting protein (PPYR1) in Drosophila. / Kókai, Endre; Szuperák, Milán; Alphey, Luke; Gausz, János; Ádám, Géza; Dombrádi, V.

In: Gene Expression Patterns, Vol. 6, No. 7, 10.2006, p. 724-729.

Research output: Contribution to journalArticle

Kókai, Endre ; Szuperák, Milán ; Alphey, Luke ; Gausz, János ; Ádám, Géza ; Dombrádi, V. / Germ line specific expression of a protein phosphatase Y interacting protein (PPYR1) in Drosophila. In: Gene Expression Patterns. 2006 ; Vol. 6, No. 7. pp. 724-729.
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