Genotyping of Chlamydia trachomatis from the endocervical specimens of high-risk women in Hungary

Fruzsina Petrovay, Eszter Balla, István Németh, E. Gönczöl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The distribution of different Chlamydia trachomatis serovars in Hungary has not been reported previously. The objective of this study was to determine the distribution and prevalence of C. trachomatis serovars in a high-risk population by genotyping. The endocervical specimens of 484 female sex workers (FSWs) were screened for C. trachomatis by plasmid PCR. Genotyping was performed in all C. trachomatis-positive samples by PCR-based RFLP analysis of the omp1 gene. A total of 32 specimens (6.6 %) were positive for C. trachomatis. Age was an important risk factor for C. trachomatis infection in FSWs. The highest prevalence was detected in women under the age of 20 (18.8 %). All positive specimens were successfully genotyped and seven serovars were identified. The most prevalent was serovar D (34.4 %), followed by E (21.9 %), F (18.8 %), G (9.4 %), J (9.4 %), H (3.1 %) and I (3.1 %). A heterogeneous distribution of C. trachomatis serovars was observed in the study group, where the most common serovars were D, E and F comprising 75% of the positive samples. This PCR-based RFLP method could be used in epidemiological studies on the prevalence of C. trachomatis infection to provide more information and to compare the serovar distribution among different cohorts.

Original languageEnglish
Pages (from-to)760-764
Number of pages5
JournalJournal of Medical Microbiology
Volume58
Issue number6
DOIs
Publication statusPublished - Jun 2009

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Hungary
Chlamydia trachomatis
Chlamydia Infections
Sex Workers
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Serogroup
Epidemiologic Studies
Plasmids

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Genotyping of Chlamydia trachomatis from the endocervical specimens of high-risk women in Hungary. / Petrovay, Fruzsina; Balla, Eszter; Németh, István; Gönczöl, E.

In: Journal of Medical Microbiology, Vol. 58, No. 6, 06.2009, p. 760-764.

Research output: Contribution to journalArticle

Petrovay, Fruzsina ; Balla, Eszter ; Németh, István ; Gönczöl, E. / Genotyping of Chlamydia trachomatis from the endocervical specimens of high-risk women in Hungary. In: Journal of Medical Microbiology. 2009 ; Vol. 58, No. 6. pp. 760-764.
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