Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks

Vito Martella, Gabriella Elia, Maria Stella Lucente, Nicola Decaro, Eleonora Lorusso, K. Bányai, Merete Blixenkrone-Møller, Nguyen Thi Lan, Ryoji Yamaguchi, Francesco Cirone, Leland Eugene Carmichael, Canio Buonavoglia

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-naïve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease.

Original languageEnglish
Pages (from-to)32-42
Number of pages11
JournalVeterinary Microbiology
Volume122
Issue number1-2
DOIs
Publication statusPublished - May 16 2007

Fingerprint

Canine Distemper Virus
Canine distemper virus
Multiplex Polymerase Chain Reaction
genotyping
Disease Outbreaks
Polymerase Chain Reaction
Vaccines
vaccines
disease control
Genotype
Dog Diseases
genotype
dog diseases
lethal genes
carnivores
epidemiological studies
epidemiology
Arctic region
Epidemiologic Studies
Epidemiology

Keywords

  • Canine distemper virus
  • Characterization
  • Protein H
  • RT-PCR

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. / Martella, Vito; Elia, Gabriella; Lucente, Maria Stella; Decaro, Nicola; Lorusso, Eleonora; Bányai, K.; Blixenkrone-Møller, Merete; Lan, Nguyen Thi; Yamaguchi, Ryoji; Cirone, Francesco; Carmichael, Leland Eugene; Buonavoglia, Canio.

In: Veterinary Microbiology, Vol. 122, No. 1-2, 16.05.2007, p. 32-42.

Research output: Contribution to journalArticle

Martella, V, Elia, G, Lucente, MS, Decaro, N, Lorusso, E, Bányai, K, Blixenkrone-Møller, M, Lan, NT, Yamaguchi, R, Cirone, F, Carmichael, LE & Buonavoglia, C 2007, 'Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks', Veterinary Microbiology, vol. 122, no. 1-2, pp. 32-42. https://doi.org/10.1016/j.vetmic.2007.01.005
Martella, Vito ; Elia, Gabriella ; Lucente, Maria Stella ; Decaro, Nicola ; Lorusso, Eleonora ; Bányai, K. ; Blixenkrone-Møller, Merete ; Lan, Nguyen Thi ; Yamaguchi, Ryoji ; Cirone, Francesco ; Carmichael, Leland Eugene ; Buonavoglia, Canio. / Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. In: Veterinary Microbiology. 2007 ; Vol. 122, No. 1-2. pp. 32-42.
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