Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation

Ksenija Lopandic, Walter P. Pfliegler, Wolfgang Tiefenbrunner, Helmut Gangl, M. Sipiczki, Katja Sterflinger

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusAccepted/In press - Apr 13 2016

Fingerprint

Fermentation
Saccharomyces cerevisiae
Yeasts
Genome
Saccharomyces
Clone Cells
Spores
Chromosomes
Meiosis
Wine
Genetic Markers
Glycerol
Ethanol
Genotype
Population

Keywords

  • AFLP
  • Genotypic and phenotypic evolution
  • High-sugar wine fermentation
  • Karyotyping
  • Saccharomyces interspecies hybrids

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation. / Lopandic, Ksenija; Pfliegler, Walter P.; Tiefenbrunner, Wolfgang; Gangl, Helmut; Sipiczki, M.; Sterflinger, Katja.

In: Applied Microbiology and Biotechnology, 13.04.2016, p. 1-13.

Research output: Contribution to journalArticle

Lopandic, Ksenija ; Pfliegler, Walter P. ; Tiefenbrunner, Wolfgang ; Gangl, Helmut ; Sipiczki, M. ; Sterflinger, Katja. / Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation. In: Applied Microbiology and Biotechnology. 2016 ; pp. 1-13.
@article{d5395378500f447e8896c60c2c60642e,
title = "Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation",
abstract = "The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.",
keywords = "AFLP, Genotypic and phenotypic evolution, High-sugar wine fermentation, Karyotyping, Saccharomyces interspecies hybrids",
author = "Ksenija Lopandic and Pfliegler, {Walter P.} and Wolfgang Tiefenbrunner and Helmut Gangl and M. Sipiczki and Katja Sterflinger",
year = "2016",
month = "4",
day = "13",
doi = "10.1007/s00253-016-7481-0",
language = "English",
pages = "1--13",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation

AU - Lopandic, Ksenija

AU - Pfliegler, Walter P.

AU - Tiefenbrunner, Wolfgang

AU - Gangl, Helmut

AU - Sipiczki, M.

AU - Sterflinger, Katja

PY - 2016/4/13

Y1 - 2016/4/13

N2 - The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.

AB - The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.

KW - AFLP

KW - Genotypic and phenotypic evolution

KW - High-sugar wine fermentation

KW - Karyotyping

KW - Saccharomyces interspecies hybrids

UR - http://www.scopus.com/inward/record.url?scp=84963681282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963681282&partnerID=8YFLogxK

U2 - 10.1007/s00253-016-7481-0

DO - 10.1007/s00253-016-7481-0

M3 - Article

C2 - 27075738

AN - SCOPUS:84963681282

SP - 1

EP - 13

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

ER -