Genotypic and phenotypic characterisation of invasive Streptococcus pneumoniae isolates from Hungary, and coverage of the conjugate vaccines

Orsolya Dobay, Ágnes Ungvári, Szilvia Kardos, Katalin Kristóf, Edit Hajdú, Judit Szabó, Márta Knausz, Erzsébet Nagy, Ferenc Rozgonyi, Sebastian G.B. Amyes, Károly Nagy

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6 Citations (Scopus)

Abstract

Background and aims: The 7-valent conjugate pneumococcal vaccine (Prevenar) was introduced as a recommended (but not yet obligatory) vaccine in Hungary in April 2009 and there was a sharp increase in the number of children vaccinated. Hence there is an urgent need for in-depth epidemiological data on invasive pneumococci before vaccination becomes widespread. Such a study has never been done before in Hungary. Methods: 144 pneumococcal isolates, obtained from invasive infections or pneumonia, were collected from eight Hungarian diagnostic laboratories between 2000 and 2008. After confirmation of species identity, their susceptibilities to nine antibiotics were determined by Etest and agar dilution method. The serotypes and pulsed-field gel electrophoresis genotypes of the strains were also determined. Results: In this cohort, most of the isolates were from patients at the extreme of life. Only 1.4% of the strains were resistant to penicillin, but nearly 40% were resistant to erythromycin (mainly due to erm(B) gene). Higher incidences of resistance were found in the very young and very old. The most prevalent serotypes in the cohort in descending order were 14, 6A, 6, 6B, 23F, 3, 19F and 11A. Conclusions: Results showed a similar but not identical profile to previously examined strains causing pulmonary infections in Hungary. The serotypes could be correlated to patient groups. Furthermore, there were examples of serotype switching in strains showing identical genotype but different serotype. The study also shows a good coverage by the conjugate vaccines over the invasive pneumococcal strains in Hungary based on the detected serotypes.

Original languageEnglish
Pages (from-to)1116-1120
Number of pages5
JournalJournal of Clinical Pathology
Volume63
Issue number12
DOIs
Publication statusPublished - Dec 1 2010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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