Genomic characterization of a novel group a lamb rotavirus isolated in Zaragoza, Spain

Max Ciarlet, Christian Hoffmann, Eleonora Lorusso, Rafael Baselga, Maria Assunta Cafiero, K. Bányai, Jelle Matthijnssens, Viviana Parreño, Simona De Grazia, Canio Buonavoglia, Vito Martella

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8*trypsin- cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95-98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

Original languageEnglish
Pages (from-to)250-265
Number of pages16
JournalVirus Genes
Volume37
Issue number2
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Rotavirus
Spain
New World Camelids
Genotype
Amino Acids
Antigenic Variation
Gastroenteritis
Ruminants
Trypsin
Italy
Genes
Sheep

Keywords

  • Gastroenteritis
  • Genetic diversity
  • Genome
  • Genotype
  • Lamb
  • NSP4
  • NSP5
  • Reassortment
  • Rotavirus
  • VP4
  • VP6
  • VP7

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Ciarlet, M., Hoffmann, C., Lorusso, E., Baselga, R., Cafiero, M. A., Bányai, K., ... Martella, V. (2008). Genomic characterization of a novel group a lamb rotavirus isolated in Zaragoza, Spain. Virus Genes, 37(2), 250-265. https://doi.org/10.1007/s11262-008-0257-6

Genomic characterization of a novel group a lamb rotavirus isolated in Zaragoza, Spain. / Ciarlet, Max; Hoffmann, Christian; Lorusso, Eleonora; Baselga, Rafael; Cafiero, Maria Assunta; Bányai, K.; Matthijnssens, Jelle; Parreño, Viviana; De Grazia, Simona; Buonavoglia, Canio; Martella, Vito.

In: Virus Genes, Vol. 37, No. 2, 10.2008, p. 250-265.

Research output: Contribution to journalArticle

Ciarlet, M, Hoffmann, C, Lorusso, E, Baselga, R, Cafiero, MA, Bányai, K, Matthijnssens, J, Parreño, V, De Grazia, S, Buonavoglia, C & Martella, V 2008, 'Genomic characterization of a novel group a lamb rotavirus isolated in Zaragoza, Spain', Virus Genes, vol. 37, no. 2, pp. 250-265. https://doi.org/10.1007/s11262-008-0257-6
Ciarlet, Max ; Hoffmann, Christian ; Lorusso, Eleonora ; Baselga, Rafael ; Cafiero, Maria Assunta ; Bányai, K. ; Matthijnssens, Jelle ; Parreño, Viviana ; De Grazia, Simona ; Buonavoglia, Canio ; Martella, Vito. / Genomic characterization of a novel group a lamb rotavirus isolated in Zaragoza, Spain. In: Virus Genes. 2008 ; Vol. 37, No. 2. pp. 250-265.
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