Genomewide screen for modulators of evolvability under toxic antibiotic exposure

Orsolya Méhi, Balázs Bogos, Bálint Csörgo, C. Pál

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Antibiotic resistance is generally selected within a window of concentrations high enough to inhibit wild-type growth but low enough for new resistant mutants to emerge. We studied de novo evolution of resistance to ciprofloxacin in an Escherichia coli knockout library. Five null mutations had little or no effect on intrinsic antibiotic susceptibility but increased the upper antibiotic dosage to which initially sensitive populations could adapt. These mutations affect mismatch repair, translation fidelity, and iron homeostasis.

Original languageEnglish
Pages (from-to)3453-3456
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume57
Issue number7
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Poisons
Anti-Bacterial Agents
Mutation
DNA Mismatch Repair
Vulnerable Populations
Ciprofloxacin
Microbial Drug Resistance
Libraries
Homeostasis
Iron
Escherichia coli
Growth

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Genomewide screen for modulators of evolvability under toxic antibiotic exposure. / Méhi, Orsolya; Bogos, Balázs; Csörgo, Bálint; Pál, C.

In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 7, 07.2013, p. 3453-3456.

Research output: Contribution to journalArticle

Méhi, Orsolya ; Bogos, Balázs ; Csörgo, Bálint ; Pál, C. / Genomewide screen for modulators of evolvability under toxic antibiotic exposure. In: Antimicrobial Agents and Chemotherapy. 2013 ; Vol. 57, No. 7. pp. 3453-3456.
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