Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera

Laura Miozzi, Giorgio Gambino, J. Burgyán, Vitantonio Pantaleo

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In plants, RNA silencing is a surveillance mechanism against invading viruses. It involves the production of virus-derived small interfering RNAs (vsiRNAs), which guide the RNA-induced silencing complex (RISC) to inactivate viruses. vsiRNAs may also promote the silencing of host mRNAs in a sequence-specific manner. In this work, vsiRNAs derived from two grapevine-infecting viruses (Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus) were selected from cDNA libraries of short RNAs and were cross-referenced with the remnants of both cleaved host transcripts and viral RNAs from a degradome dataset. We identified dozens of host transcripts targeted by vsiRNAs. Among them, several encode putative proteins involved in ribosome biogenesis and in biotic and abiotic stresses. Moreover, we identified vsiRNAs which explain the cleavage sites in viral genomes. A consistent fraction of vsiRNAs did not apparently account for cleavage, suggesting that only a low percentage of vsiRNAs are involved in the antiviral response.

Original languageEnglish
Pages (from-to)30-43
Number of pages14
JournalMolecular Plant Pathology
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Vitis
Vitis vinifera
virus
RNA
genome
small interfering RNA
Genome
Viruses
viruses
Small Interfering RNA
Grapevine fleck virus
Grapevine rupestris stem pitting-associated virus
cleavage
RNA-Induced Silencing Complex
Guide RNA
Plant RNA
Satellite Viruses
Viral Genome
biotic stress
ribosomes

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Soil Science
  • Molecular Biology

Cite this

Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera. / Miozzi, Laura; Gambino, Giorgio; Burgyán, J.; Pantaleo, Vitantonio.

In: Molecular Plant Pathology, Vol. 14, No. 1, 01.2013, p. 30-43.

Research output: Contribution to journalArticle

Miozzi, Laura ; Gambino, Giorgio ; Burgyán, J. ; Pantaleo, Vitantonio. / Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera. In: Molecular Plant Pathology. 2013 ; Vol. 14, No. 1. pp. 30-43.
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