Genome-wide identification of the glutathione transferase superfamily in the model organism Brachypodium distachyon

Ágnes Gallé, Dániel Benyó, J. Csiszár, J. Györgyey

Research output: Contribution to journalArticle

Abstract

The detoxification of harmful metabolites can determine the effectiveness of plant stress responses. Scavenging some of these toxic stress by-products through the reduced form of glutathione is catalysed by members of the glutathione transferase (GST) enzyme superfamily. The involvement of these enzymes was studied in the model organism Brachypodium distachyon (L.) P.Beauv. Bd21 and in its derivative Bd21-3, a more drought tolerant line. Osmotic stress treatment resulted in a decrease in the water potential of both Brachypodium genotypes, the difference between the control and treated plant's ψw decreased by the last sampling day in Bd21-3, suggesting some degree of adaptation to the applied osmotic stress. Increased GST activity revealed a severe defence reaction against the harmful imbalance of the redox environment. Screening for the gene sequences led to the identification of 91 full-length or partial GST sequences. Although purple false brome has a relatively small genome, the number of identified GST genes was almost as high as the number predicted in wheat. The estimation of GST expression showed stress-induced differences: higher expression levels or the fast induction of BdGSTF8, BdGSTU35 and BdGSTU42 gene products presumably indicate a strong detoxification under osmotic stress.

Original languageEnglish
Pages (from-to)1049-1062
Number of pages14
JournalFunctional Plant Biology
Volume46
Issue number11
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Brachypodium distachyon
osmotic stress
glutathione transferase
genome
organisms
Brachypodium
plant stress
enzymes
water potential
byproducts
glutathione
stress response
genes
drought
metabolites
screening
nucleotide sequences
wheat
genotype
sampling

Keywords

  • drought stress
  • drought tolerance
  • gene expression
  • glutathione S-transferase
  • osmotic stress

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Genome-wide identification of the glutathione transferase superfamily in the model organism Brachypodium distachyon. / Gallé, Ágnes; Benyó, Dániel; Csiszár, J.; Györgyey, J.

In: Functional Plant Biology, Vol. 46, No. 11, 01.01.2019, p. 1049-1062.

Research output: Contribution to journalArticle

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