Genome-wide analysis of Ollier disease: Is it all in the genes?

Twinkal C. Pansuriya, Jan Oosting, T. Krenács, Antonie H M Taminiau, Suzan H M Verdegaal, Luca Sangiorgi, Raf Sciot, Pancras C W Hogendoorn, Karoly Szuhai, Judith V M G Bovée

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Abstract

Background: Ollier disease is a rare, non-hereditary disorder which is characterized by the presence of multiple enchondromas (ECs), benign cartilaginous neoplasms arising within the medulla of the bone, with an asymmetric distribution. The risk of malignant transformation towards central chondrosarcoma (CS) is increased up to 35%. The aetiology of Ollier disease is unknown. Methods: We undertook genome-wide copy number and loss of heterozygosity (LOH) analysis using Affymetrix SNP 6.0 array on 37 tumours of 28 Ollier patients in combination with expression array using Illumina BeadArray v3.0 for 7 ECs of 6 patients. Results: Non-recurrent EC specific copy number alterations were found at FAM86D, PRKG1 and ANKS1B. LOH with copy number loss of chromosome 6 was found in two ECs from two unrelated Ollier patients. One of these patients also had LOH at chromosome 3. However, no common genomic alterations were found for all ECs. Using an integration approach of SNP and expression array we identified loss as well as down regulation of POU5F1 and gain as well as up regulation of NIPBL. None of these candidate regions were affected in more than two Ollier patients suggesting these changes to be random secondary events in EC development. An increased number of genetic alterations and LOH were found in Ollier CS which mainly involves chromosomes 9p, 6q, 5q and 3p. Conclusions: We present the first genome-wide analysis of the largest international series of Ollier ECs and CS reported so far and demonstrate that copy number alterations and LOH are rare and non-recurrent in Ollier ECs while secondary CS are genetically unstable. One could predict that instead small deletions, point mutations or epigenetic mechanisms play a role in the origin of ECs of Ollier disease.

Original languageEnglish
Article number2
JournalOrphanet Journal of Rare Diseases
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

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Enchondromatosis
Chondroma
Loss of Heterozygosity
Genome
Chondrosarcoma
Genes
Single Nucleotide Polymorphism
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 3
Sequence Deletion
Point Mutation
Epigenomics
Neoplasms
Up-Regulation
Down-Regulation
Chromosomes
Bone and Bones

ASJC Scopus subject areas

  • Medicine(all)
  • Genetics(clinical)
  • Pharmacology (medical)

Cite this

Pansuriya, T. C., Oosting, J., Krenács, T., Taminiau, A. H. M., Verdegaal, S. H. M., Sangiorgi, L., ... Bovée, J. V. M. G. (2011). Genome-wide analysis of Ollier disease: Is it all in the genes? Orphanet Journal of Rare Diseases, 6(1), [2]. https://doi.org/10.1186/1750-1172-6-2

Genome-wide analysis of Ollier disease : Is it all in the genes? / Pansuriya, Twinkal C.; Oosting, Jan; Krenács, T.; Taminiau, Antonie H M; Verdegaal, Suzan H M; Sangiorgi, Luca; Sciot, Raf; Hogendoorn, Pancras C W; Szuhai, Karoly; Bovée, Judith V M G.

In: Orphanet Journal of Rare Diseases, Vol. 6, No. 1, 2, 2011.

Research output: Contribution to journalArticle

Pansuriya, TC, Oosting, J, Krenács, T, Taminiau, AHM, Verdegaal, SHM, Sangiorgi, L, Sciot, R, Hogendoorn, PCW, Szuhai, K & Bovée, JVMG 2011, 'Genome-wide analysis of Ollier disease: Is it all in the genes?', Orphanet Journal of Rare Diseases, vol. 6, no. 1, 2. https://doi.org/10.1186/1750-1172-6-2
Pansuriya, Twinkal C. ; Oosting, Jan ; Krenács, T. ; Taminiau, Antonie H M ; Verdegaal, Suzan H M ; Sangiorgi, Luca ; Sciot, Raf ; Hogendoorn, Pancras C W ; Szuhai, Karoly ; Bovée, Judith V M G. / Genome-wide analysis of Ollier disease : Is it all in the genes?. In: Orphanet Journal of Rare Diseases. 2011 ; Vol. 6, No. 1.
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