Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis

Kinga Umenhoffer, Gábor Draskovits, Ákos Nyerges, Ildikó Karcagi, Balázs Bogos, Edit Tímár, Bálint Csörgö, Róbert Herczeg, István Nagy, Tamás Fehér, C. Pál, G. Pósfai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The ideal bacterial chassis provides a simplified, stable and predictable host environment for synthetic biological circuits. Mutability and evolution can, however, compromise stability, leading to deterioration of artificial genetic constructs. By eliminating certain sources of instability, these undesired genetic changes can be mitigated. Specifically, deletion of prophages and insertion sequences, nonessential constituents of bacterial genomes, has been shown to be beneficial in cellular and genetic stabilization. Here, we sought to establish a rapid methodology to improve the stability of microbial hosts. The novel workflow involves genome shuffling between a mobile genetic element-free strain and the target cell, and subsequent rounds of CRISPR/Cas-assisted MAGE on multiplex targets. The power and speed of the procedure was demonstrated on E. Coli BL21(DE3), a host routinely used for plasmid-based heterologous protein expression. All 9 prophages and 50 insertion elements were efficiently deleted or inactivated. Together with additional targeted manipulations (e.g., inactivation of error-prone DNA-polymerases), the changes resulted in an improved bacterial host with a hybrid (harboring segments of K-12 DNA), 9%-downsized and clean genome. The combined capacity of phage-mediated generalized transduction and CRISPR/Cas-selected MAGE offers a way for rapid, large scale editing of bacterial genomes.

Original languageEnglish
Pages (from-to)1471-1483
Number of pages13
JournalACS Synthetic Biology
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 18 2017

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Interspersed Repetitive Sequences
Prophages
Bacterial Genomes
Chassis
Stabilization
Genes
Genome
Workflow
Sequence Deletion
Insertional Mutagenesis
DNA-Directed DNA Polymerase
Bacteriophages
Plasmids
DNA
Escherichia coli
Deterioration
Proteins
Networks (circuits)

Keywords

  • bacterial genome reduction
  • CRISPR/Cas-assisted multiplex automated genome editing
  • genome shuffling
  • genome stabilization
  • mutation rate
  • transposon inactivation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis. / Umenhoffer, Kinga; Draskovits, Gábor; Nyerges, Ákos; Karcagi, Ildikó; Bogos, Balázs; Tímár, Edit; Csörgö, Bálint; Herczeg, Róbert; Nagy, István; Fehér, Tamás; Pál, C.; Pósfai, G.

In: ACS Synthetic Biology, Vol. 6, No. 8, 18.08.2017, p. 1471-1483.

Research output: Contribution to journalArticle

Umenhoffer, K, Draskovits, G, Nyerges, Á, Karcagi, I, Bogos, B, Tímár, E, Csörgö, B, Herczeg, R, Nagy, I, Fehér, T, Pál, C & Pósfai, G 2017, 'Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis', ACS Synthetic Biology, vol. 6, no. 8, pp. 1471-1483. https://doi.org/10.1021/acssynbio.6b00378
Umenhoffer, Kinga ; Draskovits, Gábor ; Nyerges, Ákos ; Karcagi, Ildikó ; Bogos, Balázs ; Tímár, Edit ; Csörgö, Bálint ; Herczeg, Róbert ; Nagy, István ; Fehér, Tamás ; Pál, C. ; Pósfai, G. / Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis. In: ACS Synthetic Biology. 2017 ; Vol. 6, No. 8. pp. 1471-1483.
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