Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid

Y. Li, Z. Zádori, H. Bando, R. Dubuc, G. Fédière, J. Szelei, P. Tijssen

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Bombyx mori densovirus (BmDNV-1), on the basis of the previously reported genome sequence, constitutes by itself a separate genus (Iteravirus) within the Densovirinae subfamily of parvoviruses. Inconsistencies in the genome organization, however, necessitated its reassessment. The genome sequence of new clones was determined and resulted in a completely different genome organization. The corrected sequence also contained conserved sequence motifs found in other parvoviruses. Some amino acids in the highly conserved domain in the unique region of VP1 were shared by critical amino acids in the catalytic site and Ca2+-binding loop of secreted phospholipase A2, such as from snake and bee venoms. Expression of this domain and determination of enzyme activity demonstrated that capsids have a phospholipase A2 activity thus far unknown to occur in viruses. This viral phospholipase A2, which is required shortly after entry into the cell, showed a substrate preference for phosphatidylethanolamine and phosphatidylcholine over phosphatidylinositol.

Original languageEnglish
Pages (from-to)2821-2825
Number of pages5
JournalJournal of General Virology
Volume82
Issue number11
Publication statusPublished - 2001

Fingerprint

Densovirus
Bombyx
Capsid
Densovirinae
Genome
Parvovirus
Phospholipases A2
Enzymes
Secretory Phospholipase A2
Bee Venoms
Amino Acids
Snake Venoms
Conserved Sequence
Phosphatidylinositols
Phosphatidylcholines
Catalytic Domain
Clone Cells
Viruses

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Li, Y., Zádori, Z., Bando, H., Dubuc, R., Fédière, G., Szelei, J., & Tijssen, P. (2001). Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid. Journal of General Virology, 82(11), 2821-2825.

Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid. / Li, Y.; Zádori, Z.; Bando, H.; Dubuc, R.; Fédière, G.; Szelei, J.; Tijssen, P.

In: Journal of General Virology, Vol. 82, No. 11, 2001, p. 2821-2825.

Research output: Contribution to journalArticle

Li, Y, Zádori, Z, Bando, H, Dubuc, R, Fédière, G, Szelei, J & Tijssen, P 2001, 'Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid', Journal of General Virology, vol. 82, no. 11, pp. 2821-2825.
Li, Y. ; Zádori, Z. ; Bando, H. ; Dubuc, R. ; Fédière, G. ; Szelei, J. ; Tijssen, P. / Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid. In: Journal of General Virology. 2001 ; Vol. 82, No. 11. pp. 2821-2825.
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